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一株萎缩芽孢杆菌 XW-2 对松材线虫的杀线活性∗
肖海燕1, 黄华毅2, 胡铭彪2, 龙锦发2, 陈刘生2, 黄咏槐2, 赵丹阳2, 扈丽丽2
1.韶关市国有林场事务中心;2.广东省林业科学研究院
摘要:
为明确萎缩芽孢杆菌 Bacillus atrophaeus XW-2 对松材线虫 Bursaphelenchus xylophilus 的杀线活 性, 开发松材线虫病生防制剂, 研究通过室内浸渍试验, 测定了 XW-2 菌悬液和发酵上清液对松材线虫 的杀线活性。 结果表明, XW-2 的菌悬液和发酵上清液原液处理松材线虫 12 h 时, 松材线虫死亡率分别 为 100%和 91. 00%; 处理 24 h 的死亡率均可达 100%; 随着稀释倍数的增大, 线虫死亡率逐渐降低, 稀释 100 倍时, 菌悬液处理松材线虫 24 h 的线虫死亡率为 86. 67%, 发酵上清液处理的线虫死亡率为 63. 00%。 因此, XW-2 菌悬液和发酵上清液对松材线虫均具有杀线活性, 相同浓度下的菌悬液杀线活性显著 (P< 0. 05) 高于发酵上清液。
关键词:  生防制剂  松材线虫  综合防控
DOI:
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基金项目:广东省林业科技创新项目 (2023KJCX024)。
The Activity of Bacillus atrophaeus XW-2 Against Bursaphelenchus xylophilus
Xiao Hai-yan1, HUANG Hua-yi2, HU Ming-biao2, LONG Jinfa2, CHEN Liu-sheng2, HUANG Yong-huai2, ZHAO Dan-yang2, HU Li-li2
1.Shaoguan State owned Forest Farm Affairs Center;2.Guangdong Acdamy of forestry
Abstract:
To determine the killing activity of Bacillus atrophaeus XW-2 against the Bursaphelenchus xylophilus, a biocontrol agent against pine wood nematode disease was developed. The killing activity of XW-2 bacterial suspension and fermentation supernatant against Bursaphelenchus xylophilus was determined by the indoor immersion test. The results showed that when XW - 2 bacterial suspension and fermentation supernatant were used to treat pine wood nematodes for 12 hours, the correction rates for pine wood nematodes were 100% and 91. 00%, respectively. However, after 24 h of treatment, the corrected mortality rates reached 100%. As the dilution concentration increases, the mortality rate of nematodes gradually decreases. However, after diluting the bacterial suspension 100 times and treating nematodes for 24 hours, the corrected mortality rate of nematodes is 86. 67%. However, after diluting the fermentation supernatant 100 times and treating pine wood nema-todes for 24 hours, the corrected mortality rate of nematodes is 63. 00%. Therefore, the XW-2 bacterial suspension and fermentation supernatant both had bactericidal activity against pine wood nematodes, and the bactericidal activity of the bacterial susp
Key words:  biocontrol agents, pine wood nematode, prevention and control