| 摘要: |
| 为进一步鉴定红皮柳 Salix purpurea 的 SpXTHs 基因家族的生物学功能, 通过生物信息学方法
从红皮柳基因组中鉴定了 31 个 SpXTHs 基因, 并对该家族成员的理化性质、 表达模式、 进化等进行分析。
通过系统发育分析, 表明 31 个 SpXTHs 成员可分为 4 个进化分枝, 每个分枝内的基因具有相似的基因结
构; 通过分析 SpXTHs 家族成员的蛋白保守结构域, 发现 SpXTHs 家族成员均含有 Glyco_hydro_16 和 XET_C
两个蛋白保守结构域, 具有高度相似的保守基序; SpXTHs 含有 241 ~ 374 个氨基酸残基, 该蛋白家族成员
长度及分子量有较大差异, 相对分子质量约为 27 ~ 42 kDa, 等电点均为酸性。 根据全基因组分析结果,
SpXTHs 基因经历了全基因组复制事件和片段重复事件。 红皮柳与拟南芥 Arabidopsis thaliana、 甘蓝 Brassica oleracea var. capitata 和水稻 Oryza sativa 的比较同线图表明, 红皮柳与拟南芥、 甘蓝和水稻 XTHs 基
因均具有同线关系。 通过对 SpXTHs 基因启动子区域进行顺式作用元件预测, 共鉴定出 7 种顺式作用元
件。 在 3%NaCl 溶液处理后, 红皮柳根部共有包括 SpXTH1、 SpXTH5 等 7 个基因高表达。 该研究鉴定了红
皮柳 SpXTHs 基因家族共计 31 个成员, 并进行了生物信息学分析, 为红皮柳 SpXTHs 蛋白功能的后续分子
机制解析提供了实验数据支撑。 |
| 关键词: 红皮柳 木葡聚糖内转葡糖基酶/ 水解酶 生物信息学 表达特征 |
| DOI： |
| 分类号: |
| 基金项目:2024年中国科协定点帮扶第一批项目(2024ddbfxczx-04) |
|
| Identification and Expression Analysis of the SpXTHs Gene Family in Salix purpurea |
|
Hu Zhibao1, Jing Shuo1, Lin Yang1, Shen Yinan1, Hu Fangyuan1, Zhang Haokai1, Wang Hongjiang2
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1.Liaoning Institute of Agriculture& Forestry in Arid Areas;2.Liaoning Institute of Agriculture Forestry in Arid Areas,Chaoyang,Liaoning
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| Abstract: |
| In order to further identify the biological functions of the SpXTHs gene family of Salix purpurea,
31 SpXTHs genes were identified from the genome of S. purpurea through bioinformatics methods, and the physical and chemical properties, expression patterns, and evolution of the family members were analyzed. Phylogenetic analysis showed that the 31 SpXTHs members could be divided into four evolutionary branches, and the
genes in each branch had similar gene structures. By analyzing the protein conserved domains of SpXTHs family
members, it was found that all SpXTHs family members contained two protein conserved domains, Glyco_hydro_16 and XET_C, and had highly similar conserved motifs; SpXTHs contained 241-374 amino acid residues. Members of this protein family vary greatly in length and molecular weight. The molecular weight is
about 27~42 kDa, and the isoelectric point is acidic. Based on the whole-genome analysis, the SpXTH genes
underwent whole-genome duplication events and segmental duplication events. Comparative synteny analysis
revealed that S. purpurea shares syntenic relationships with XTHs genes in Arabidopsis thaliana, Brassica oleracea var. capitata, and Oryza sativa. Prediction of cis-acting elements in the promoter regions of SpXTH genes
identified seven types of cis - acting regulatory elements. Following treatment with 3% NaCl, seven genes,
including SpXTH1 and SpXTH5, exhibited significantly high expression levels in the roots of S. purpurea. In
this study, a total of 31 members of the SpXTHs gene family of S. purpurea were identified and bioinformatics
analysis was carried out, which provided important information for further identification of the biological function of S. purpurea SpXTHs proteins. |
| Key words: Salix purpurea xyloglucan endotransglucosylase / hydrolase bioinformatics expression profile |
