| 摘要: |
| 为了解龙脑含量的高低对阴香 Cinnamomum burmanni
响, 文章以
叶不同发育阶段叶际细菌群多样性的影
2 个阴香品系 (高龙脑含量—H 和低含量—L) 的 2 个发育时期叶片 (嫩叶和成熟叶) 为研究
材料, 利用气相-质谱联用仪检测 4 组样本的龙脑含量, 利用 16S 测序结果比较分析叶际细菌结构和相对
丰度的变化。 结果表明: (1) 相较于嫩叶, 2 个品系的成熟叶中龙脑含量均显著增加。 (2) 4 组样本共检
测出 259 个操作分类单元, 其中 H 特有操作分类单元的数量最多。 (3) 基于这些 H 成熟叶的菌群 α 多样
性指数在 4 组样本中最高, 高龙脑含量品系的叶片中叶际细菌群落多样性随着叶片成熟而提升, 低龙脑含
量品系的叶际细菌群落多样性随着叶片成熟变化并不显著。 4 组样本之间菌群丰度的差异主要集中在鞘氨
醇单胞菌属菌 Sphingomonas sp. 、 短小杆菌属菌 Curtobacterium sp. 、 假单胞菌属菌 Pseudomonas sp. 、 肠
杆菌科菌 Enterobacteriaceae sp. 、 类芽孢杆菌属菌 Paenibacillus sp. 和芽孢杆菌属菌 Bacillus sp. 。 研究结
果表明阴香叶中龙脑含量的变化影响了叶际细菌菌群结构和丰度, 阴香叶片中的龙脑含量越高则叶际细
菌菌群多样性随着叶片成熟后越容易发生变化。 |
| 关键词: 阴香 龙脑 叶际细菌 生物多样性 |
| DOI： |
| 投稿时间：2024-02-24修订日期：2024-04-08 |
| 基金项目:广东省林业科技创新项目（2023KYXM08）；广东省林业科技创新项目（编号2022KJCX006） |
|
| The Effect of Cinnamomum burmanni Content on the Diversity of Phyllospheric Microorganism |
| Zhang Hongbo,Li Bing,Zhong Yonglin,Zhang Qian,He Boxiang,Wang Yingli,Hou Chen,Yin Zuoyun |
| (Zhongkai University of Agriculture and Engineering;Guangdong Academy of Forestry) |
| Abstract: |
| There are few reports on the effect of borneol content on the biodiversity of phyllospheric microorganisms in the leaves of borneol-type Cinnamomum burmanni at different developmental stages. Accordingly,
in this paper, the leaves of two C. burmanni varieties (high borneol content-H and low borneol content-L) at
two developmental stages (tender and mature) were used as the study materials, and the content of borneol was
detected in four groups of samples by gas chromatography-mass spectrometry (GC-MS), and the results of 16S
sequencing were used to comparatively analyze the changes in the structure and relative abundance of the phyllo-spheric microorganisms. The results showed that (1) the content of borneol in mature leaves of both strains significantly increased compared to tender leaves. (2) A total of 259 operational taxonomic units (OTUs) were
detected in the 4 sets of samples, with the highest number of H-specific OTUs. (3) The α-diversity index of
the bacterial community based on these H mature leaves was the highest among the four groups of samples, and
the diversity of the phyllospheric microorganisms in the leaves of the high borneol -content varieties increased
with leaf maturity, while the diversity of the phyllospheric microorganisms in the low borneol-content lines did
not change significantly. The differences in bacterial abundance among the four groups of samples were mainly
focused on Sphingomonas sp. , Curtobacterium sp. , Pseudomonas sp. , Enterobacteriaceae sp. , Paenibacillus
sp. and Bacillus sp. . The research results indicate that changes in the content of borneol in C. burmanni leaves
affect the structure and abundance of bacterial communities in the leaf area. The higher the content of borneol in
C. burmanni leaves, the easier it is for the diversity of bacterial communities in the leaf area to change as the mature leaves. |
| Key words: Cinnamomum burmanni borneol interleaf bacteria biodiversity |
