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真菌诱导白木香所结沉香质量分析
黄世芳1, 黄满勤2, 余玉珠1, 符韵林2, 刘学林1, 邓力1
1.广西壮族自治区国有钦廉林场;2.广西大学林学院
摘要:
为探究真菌诱导白木香 Aquilaria sinensis 结香 7 个月所产生沉香的主要化学成分及其质量是否 符合行业标准, 以石湾和星岛湖两地真菌诱导结香所产生的沉香为研究对象, 测定其醇溶性提取物的含 量, 观察显色反应、 采用高效液相分析其指纹图谱, 利用气相色谱质谱联用仪 (GC-MS) 分析其主要化 学成分。 结果表明: 石湾和星岛湖的沉香醇溶性提取物分别为 (19. 29±8. 30)%、 (22. 54±5. 96)%, 显色 反应呈现紫红色和淡紫色, 高效液相特征图谱出现 6 个特征峰, 符合沉香行业标准; 石湾、 星岛湖沉香分 别具有 12, 15 个倍半萜类物质, 占 30. 80%、 30. 06%, 分别具有 4, 5 个 2- (2-苯乙基) 色酮类物质, 占 24. 81%、 30. 62%。 研究利用真菌结香剂诱导石湾和星岛湖两地的白木香结香, 结香 7 个月的沉香可以 达到 《沉香》 ( LY / T
关键词:  白木香  沉香  气相色谱质谱联用仪  化学成分
DOI:
分类号:
基金项目:广西林业科技项目“白木香高效生物结香技术研究”(No.桂林科研[2022ZC]第 91 号)。
Quality Analysis of Agarwood Produced by Fungal in Duction of Aquilaria sinensis
Huang shifang1, Huang manqin1, Yu yuzhu1, Fu yunlin2, Liu xuelin1, Deng li1
1.Guangxi state-owned Qinlian Forestry, Qinzhou, Guangxi;2.Guangxi University, College of Forestry
Abstract:
To investigate whether the quality of agarwood produced after 7 months of fungal inducer Aquilaria sinensis meets industry standards and its main chemical components. The study focuses on agarwood induced by fungi in Shiwan and Xingdaohu for 7 months. The content of its alcohol-soluble extract was determined, its color reaction was observed, its fingerprint was analyzed using high-performance liquid chromatography, and its main chemical components were analyzed using gas chromatography-mass spectrometry (GCMS). The alcohol - soluble extracts of agarwood from Shiwan and Xingdaohu were ( 19. 29 ± 8. 30)% and (22. 54 ± 5. 96)%, respectively. The chromogenic reactions showed purplish red and light purple, and the high -performance liquid chromatography characteristic spectrum also showed 6 characteristic peaks, which met the agarwood industry standards; Shiwan and Xingdaohu agarwood had 12 and 15 Sesquiterpene, accounting for 30. 80% and 30. 06% respectively, 4 and 5 2 - ( 2 - phenylethyl) chromones, accounting for 24. 81% and 30. 62% respectively. In this study, the agarwood of Shiwan and Xingdaohu for 7 months induced by fungal inducer can meet the industry standard of agarwood, and the content of Sesquiterpene and 2- (2-phenylethyl) chromones can reach 55. 61% and 60. 08%, providing new theory and method for artificial induction of agarwood. K
Key words:  Aquilaria sinensis (Lour.) Spreng  agarwood  gas chromatography-mass spectrometry  chemical components