| 摘要: |
| 以木荷 Schima superba 优树嫁接苗萌发嫩枝为插穗，分析了扦插生根过程中插穗解剖构造以及
内源激素水平的变化。结果表明：普通木荷插穗（生根率 ≈40%）无潜伏根原基，扦插 10 天时诱导产生
不定根原始体，但插穗表观形态无明显改变，而扦插 20 天时产生不定根原基、茎基部明显愈伤化膨大，
扦插 50 天不定根分化完成。与难生根基因型（生根率 <10%）相比，易生根基因型（生根率 >80%）插穗
中的 IAA 水平明显偏高，而 ZR、GA、ABA 无明显差异；扦插 20 天，插穗 IAA 较高，GA 偏低；扦插
50 天，IAA、ABA 较高，而 ZR、GA 较低。在 0~100 mg/L GA 合成抑制剂多效唑作用下，普通插穗生根
率差异显著。其中，以 50 mg/L 多效唑处理下生根率最高，达 90.1%，较对照提升了 123.6%。研究认为，
木荷属生根困难树种，内源性 IAA、GA 水平在不定根形成中起到关键作用，通过外源喷施多效唑能有效
提高木荷生根能力，但鉴于 GA 在生根过程中的双重性作用，其施用浓度需控制在合适范围。 |
| 关键词: 木荷 不定根 内源激素 生长素 赤霉素 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Study on Cutting Rooting Technology of Schima superba Based on Endogenous Hormone Regulation |
|
Wen Hanping1, Chen Jiayu1, Zhong Yuting2, Zou Guoyue2, Wu Minghua3
|
|
1.Zijin County Ecological public welfare Forest Management Center;2.Zijin Forestry Technology Extension Station;3.South China Agricultural University
|
| Abstract: |
| The changes in anatomical structure and endogenous hormone level of cuttings in the process
of rooting were analyzed by using the sprouted twigs of grafted seedlings of Schima superba as cuttings, to
provide a theoretical reference for the construction of an efficient rooting technology system of S. superba.
The results showed that there was no latent root primordium in general cuttings (rooting rate ≈ 40%) of S.
superba. Adventitious root primordia were induced at 10 days of cutting, but there was no significant change
in the apparent morphology of cuttings. Adventitious root primordia and stem base were significantly callused
and expanded at 20 days of cutting, and adventitious root differentiation was completed at 50 days of cutting.
Compared with the difficult rooting genotype (rooting rate < 10%), the IAA level in the cuttings of the easy
rooting genotype (rooting rate > 80%) was significantly higher, but there was no significant difference in ZR, GA,
and ABA. Cutting 20 days, cuttings IAA higher, GA lower; Cutting 50 days, IAA, ABA higher, while ZR, GA lower. Under the effect of 0-100 mg/L GA synthesis inhibitor, the rooting rate of general cuttings was significantly
different. Among them, the rooting rate was the highest under the treatment of 50 mg/L paclobutrazol, reaching
90.1%, which increased by 123.6% compared with the control. The results showed that the endogenous IAA
and GA levels played a key role in the formation of adventitious roots in S. superba, which was difficult to root.
Spraying exogenous paclobutrazol could effectively improve the rooting ability of S. superba, but because of the
dual role of GA in the rooting process, its application concentration should be controlled in the appropriate range. |
| Key words: Schima superba adventitious root endogenous hormone auxin gibberellic acid |
