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马尾松叶片挥发物组成及其对松墨天牛有触角电生理反 应的活性成分鉴定
赖略1, 邹学冰2, 徐金柱2, 秦长生3, 杨华3, 田龙艳3, 张春花3, 邱华龙3
1.河源市森林病虫害防治服务中心;2.河源市源城区林业局;3.广东省林业科学研究院/广东省森林培育与保护利用重点实验室
摘要:
为了明确马尾松 Pinus massoniana 叶片中吸引松墨天牛 Monochamus alternatus 的特定挥发性 化合物，利用 Y 型嗅觉仪测试了马尾松挥发物对松墨天牛的趋性，应用 GC-MS 分析鉴定了马尾松叶片中 挥发物的组成，并使用 GC-EAD 筛选了能引起松墨天牛触角电生理反应的活性化合物。结果表明：松墨 天牛对马尾松挥发物具有较强的选择趋性；GC-MS 分析表明马尾松叶片中共有 22 个化合物，其中萜烯 类物质 14 种，醇类物质 5 种，羧酸类物质和酯类物质各 1 种，以及 1 个未鉴定的化合物。在上述挥发物 中，β- 蒎烯的相对含量最高，为（28.68±0.67）%，其次为 α- 蒎烯，为（21.03±5.08）%；α- 蒎烯、 β- 蒎烯、β- 水芹烯、β- 石竹烯、D- 吉玛烯以及乙酸龙脑酯 6 个化合物能引起松墨天牛雌雄成虫的触 角电生理反应。
关键词:  松墨天牛  EAG  挥发物  萜烯化合物
DOI：
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基金项目:河源市科技计划项目（河科 2021005)
Identification of Leaf Volatiles from Pinus massoniana and Antennal Electrophysiological Activity of Volatiles to Monochamus alternatus
Lai Lue1, zou xuebing1, xu jinzhu2, qin changsheng3, yanghua3, TIANLONGYAN3, ZHANG CHUNHUA3, Qiu Hua Long3
1.//;2.Forestry Bureau of Yuancheng District of Heyuan City;3.Guangdong Academy of Forestry
Abstract:
In order to identify the specific volatile compounds that attract the Japanese pine sawyer, Monochamus alternatus Hope, 1842 (Coleoptera: Cerambycidae), in the leaves of the Masson’s pine, Pinus massoniana, the Y-type olfactometer was used to determine the attractive effect of volatile compounds of P. massoniana leaves and the composition in leaf volatiles of P. massoniana was analyzed and identified by GC-MS, as well as the active compounds that can cause the electrophysiological response of the antennae of Japanese pine sawyers were screened by GC-EAD. The results showed that the volatiles of Masson’s pine could significantly attract adults of Japanese pine sawyers . GC-MS results showed that there were 22 compounds in the Masson’s pine leaves, including 14 terpenoids, 5 alcohols, 1 carboxylic acid and 1 ester, as well as one unidentified compound. Among the leafy volatiles, the relative percentage of β-pinene was the highest (28.68 ± 0.67)%, followed by α-pinene (21.03 ± 5.08)%; Six compounds of α- Pinene, β-Pinene, β-Carylene, β-Dianthus caryophyllene, D-gemonene and bornyl acetate could elicit the antennal electrophysiological responses of male and female adults of the pine sawyer. These results will provide a reference for the chemical mechanism of M. alternatus location for the host plant and the improvement of the attractant against Japanese pine sawyers.
Key words:  Monochamus alternatus  Pinus massoniana  EAG  Volatiles  Terpenoids