| 摘要: |
| 橙带蓝尺蛾 Milionia basalis 主要危害罗汉松属和陆均松属植物，目前针对该虫的防治研究较少。为了寻找适用于该虫的防治方法，研究采用喷雾法从8 株昆虫病原菌中筛选出3 株对其幼虫的高毒力绿僵菌菌株，M09、Mac985和Ma3297。室内生物测定结果表明，用1×108 孢子/mL的孢悬液处理橙带蓝尺蛾2~3龄幼虫8 d后，3 株绿僵菌的校正死亡率均分别为98.72%、96.15%、88.46%，致死中时间LT50分别为4.043、4.226、4.868 d，致死中浓度LC50分别为1.64×104 孢子/mL、1.62×106 孢子/mL和7.95×104 孢子/mL。绿僵菌M09与高效氯氟氰菊酯和甲氨基阿维菌素苯甲酸盐混配后表现出增效作用。 |
| 关键词: 橙带蓝尺蛾 绿僵菌 毒力 协同作用 |
| DOI： |
| 投稿时间：2021-08-17修订日期：2021-08-17 |
| 基金项目: |
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| Synergism of High Virulence Strains and Different Pesticides Against the Milionia basalis |
| CHEN Zhiyun,QIN Changsheng,WANG Ling,XU Jinzhu,LI Dongwen,MO Xian,ZHAO Danyang,YANG Hua |
| (Forest?Pest?Management?and?Quarantine?Station?of?Zhongshan?City;Guangdong Academy of Forestry) |
| Abstract: |
| Milionia basalis is an important insect pest of Podocarpus and Dacrydium, and there are few studies on its control. In order to find a suitable control method for this insect, three strains of M09, Mac985 and Ma3297 with high virulence to its larvae were screened from eight insect pathogens by spray method. The results of laboratory bioassay showed that after 8 days of treatment with 1×108 conidia /mL spore suspension, the corrected mortality of the three Metarhizium against M. basalis 2nd-3rd instar larvae were 98.72%, 96.15%, 88.46%, respectively, and the the median lethal time (LT50 )were 4.043, 4.226, 4.868 d, respectively. Lethal medium concentration (LC50) was 1.64×104 conidia /mL , 1.62×106 conidia /mL and 7.95×104 conidia /mL, respectively. The mixture of spore suspension of M09 and Lambda cyhalothrin or Emamectin benzoate against the 2nd-3rd instar larvae was more effective than that of M.anisopliae alone,displaying cleary synergetic activity. |
| Key words: Milionia basalis Metarhizium spp. virulence synergy |
