| 摘要: |
| 为了更好地利用薯莨 Dioscorea cirrhosa 资源，减少薯莨渣浪费，从薯莨和薯莨渣中提取生物活性成分，比较了薯莨提取物对几种重要植物病原菌的抑菌作用。结果显示，60% 丙酮浸提法比 75% 乙醇提取法能获得较多提取物，且提取物中单宁含量较高，适合用于薯莨单宁提取。相同提取条件下，薯莨渣的浸提物得率比薯莨低 50%，但两种提取物中单宁含量无明显差异。抑菌试验结果表明，薯莨的丙酮提取物对来自猴耳环 Pithecellobium clypearia 的炭疽病菌（暂定名为 H30，Colletotrichum sp.）、拟茎点霉（暂定名为 H63，Phomopsis sp.）和广藿香青枯病菌（暂定名为 GHB2，Ralstonia sp.）均有明显的抑制效果。因此，薯莨和薯莨渣可望成为研制新型植物源杀菌剂的原料，为药用植物病害绿色防控提供新制剂。 |
| 关键词: 薯莨 单宁 杀菌剂 |
| DOI： |
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| 基金项目:广东省教育厅特色创新项目（2018GKTSCX006），广东省林业科技创新项目（2019kjcx022） |
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| Tannic Acid Extraction From Dioscorea cirrhosa and Antimicrobial Activity of D. cirrhosa Extract |
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Li Luo Ye1, Yang Xiao Zhu1, Lei Meng Ying1, Liang Chuan Guan2, He Xu Jun1, Huang Shao Bin1
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1.Guangdong Eco-Engineering Polytechnic;2.Huizhou Taihe Xinchuang Technology Co., Ltd.
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| Abstract: |
| For fully utilization of Dioscorea cirrhosa and its residues from the manufacturing process of Gambiered Guangdong silk, biologically active substance was tried to be extracted and the antimicrobial activity of D. cirrhosa extracts was tested. The results showed that more extracts were obtained and more tannic was in the extracts by 60% acetone than by 75% ethanol. It is suitable to extract tannic from D. cirrhosa using 60% acetone. Using the same extraction method, the output rate of extracts from D. cirrhosa residues was 50% lower than from D. cirrhosa, but the tannic acid content in extracts from both of them were very similar. The extracts of D. cirrhosa. from 60% acetone showed high antimicrobial activity to a species of Colletotrichum and a species of Phomopsis from Pithecellobium clypearia. It also had obvious antibacterial activity to Ralstonia solanacearum of Pogostemon cablin. So, It is possible to develop a new botanical germicide using D. cirrhosa and its residues and provide a new preparation for nuisanceless control of medicinal plant diseases. |
| Key words: Dioscorea cirrhosa tannic acid germicide |
