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红锥茎段外植体消毒和初代培养
廖焕琴,叶芷华,潘文,张卫华
广东省林业科学研究院/广东省森林培育与保护利用重点实验室 广东 广州,广东生态工程职业学院 广东 广州,广东省林业科学研究院/广东省森林培育与保护利用重点实验室 广东 广州,广东省林业科学研究院/广东省森林培育与保护利用重点实验室 广东 广州
摘要:
红锥(Castanopsis hystrix) 是华南地区重要的优质乡土阔叶树种，研究以红锥优树茎段为材料，研 究不同消毒剂及处理时间、茎段木质化程度、留叶方式、光照强度和培养基对茎段初代培养试验的影响。 结果表明，以红锥半木质化优树茎段为外植体，75% 酒精 5 s，0.1% 升汞5 min，保留1/2 叶，接种于改良 MS+1.0 mg/L 6-BA+0.2 mg/L KT+0.2 mg/L NAA 培养基，置于3 000 Lux 光照强度下培养诱导效果最佳。
关键词:  红锥  初代培养  污染率  萌芽率
DOI：
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基金项目:广东省林业科技创新项目“优良乡土阔叶树种无性快繁关键产业技术开发与产业化”（2014KJCX002）；广东省省级科技计划项目“速生优质红锥新品系选育研究” （2014B020202002）
Research on Explant Disinfection and Primary Culture of Castanopsis hystrix Stem
LIAO Huanqin,YE Zhihua,PAN Wen and ZHANG Weihua
Guangdong Academy of Forestry/ Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou,Guangdong Eco-Engineering Polytechnic,Guangzhou,Guangdong Academy of Forestry/ Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou,Guangdong Academy of Forestry/ Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou
Abstract:
Castanopsis hystrix is an important high quality native broad-leaved tree species in Southern China. This paper studied the influences of different disinfectants and treating time, maturity level, way of keeping leaves, light intensity and culture medium on primary culture of C. hystrix stem. The stems were employed as initial explants, origins of which were superior individuals. The results showed the best induction rate when the stems were treated with 75% alcohol for 5 s, 0.1% mercuric chloride for 5 min, kept 1/2 leaf, vaccinated in culture medium: MS + 1.0 mg/L 6 - BA + 0.2 mg/L KT + 0.2 mg/L NAA under 3 000 Lux light intensity.
Key words:  Castanopsis hystrix  primary culture  contamination rate  germination rate