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| 巨桉纤维素合酶Ces家族的生物信息学分析 |
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徐双,曾炳山,范春节,刘英,李湘阳,裘珍飞
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中国林业科学研究院,中国林业科学研究院,中国林业科学研究院,中国林业科学研究院,中国林业科学研究院,中国林业科学研究院
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| 摘要: |
| 纤维素合酶（Cellulose synthase，Ces）基因家族成员负责催化纤维素的合成。利用Phytozome网站对巨桉（Eucalyptus grandis）全基因组序列进行检索，共发现31条蛋白质序列属于Ces家族，其中包括18个CesA亚族成员与13个CslD亚族成员。使用ClustalW、Protparam、TMHMM等生物学在线服务器，对巨桉31个Ces家族成员进行全面生物信息学分析预测，结果显示：31条蛋白质序列均具有Cellulose synthase特征性保守结构域，具有Ces家族成员特征；巨桉Ces家族与拟南芥Ces家族、杨树Ces家族在亚族种类、成员组成、蛋白理化性质方面存在变异性；蛋白结构域、二级结构、跨膜区预测、无序性、亚细胞定位分析结果存在保守性。 |
| 关键词: 巨桉 纤维素合酶家族 生物信息学分析 |
| DOI： |
| 分类号:S718.46 |
| 基金项目:国家“863”项目“桉树与鹅掌楸转基因育种技术研究”（2013AA100705） |
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| Bioinformatics Analyses of the Ces Gene Family in Eucalyptus grandis |
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Xu Shuang,Zeng Bingshan,Fan Chunjie,Liu Ying,Li Xiangyang and Qiu Zhenfei
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Chinese Academy of Forestry,Chinese Academy of Forestry,Chinese Academy of Forestry,Chinese Academy of Forestry,Chinese Academy of Forestry
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| Abstract: |
| Cellulose synthase(hereinafter refer as Ces) is responsible for catalyzing cellulose formation. This study took advantage of the Phytozome website, examined the whole genome sequence of Eucalyptus grandis, and found 31 Ces family members in E. grandis, including 18 CesA genes and 13 CslD genes. Bioinformatic analysis using ClustalW, Protparam and TMHMM revealed characteristics of Ces family proteins and provide useful information for further functional study of E. grandis Ces genes. Results showed that these 31 protein sequences all possess the Cellulose synthase domain and shared characters with Ces family members in other tree species. However, Ces family members in E. grandis, Poplar trichocarpa and Arabidopsis thaliana had differences in number of subfamily, formation of members and physical characters, while conservation in characteristic domain, secondary structure, transmembrane region, unfoldabiligy, subcellular localization and so on. |
| Key words: Eucalyptus grandis Cellulose synthase bioinformatic analyses |
