| 摘要: |
| 采用二次正交旋转组合设计对顶果木（Acrocarpus fraxinifolius）增殖培养基进行优化，建立增殖倍数（Y）对培养基中Ca2+浓度（X1）、6BA浓度（X2）及IBA浓度（X3）的正交回归模型：Y=2.416-0.172X1-0.255X2+0.183X12-0.211X22+0.162X32+0.331X1X2。从模型可知，当Ca2+浓度为255 mg/L，6BA浓度为0.76 mg/L，IBA浓度为0.16 mg/L时，增殖倍数达最大值4.45。模型预测值与实际验证结果较接近。\ |
| 关键词: 顶果木 组织培养 增殖 二次正交旋转组合设计 |
| DOI： |
| 投稿时间：2014-03-11修订日期：2014-05-17 |
| 基金项目:广东省林业科技创新专项“顶果木引种和高效栽培技术研究与示范”（2010KJCX005） |
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| Study on Optimal Multiplication Medium of Acrocarpus fraxinifolius |
| XU Weili,XU Chaoxuan,LIN Ronghua,FU Xiaoping |
| (Zengcheng Forest Farm Guangzhou City，Guangzhou;Guangzhou Forestry Research Institute，Guangzhou) |
| Abstract: |
| The multiplication medium of Acrocarpus fraxinifolius was optimized with quadratic orthogonal rotation combination design. The quadratic orthogonal regression model of multiplication rate（Y） to three factors including concentration of Ca2 （X1）， 6BA（X2）and IBA（X3） was established as Y=2.420-0.172X1-0.255X2 0.184X12-0.211X22 0.162X32 0.331X1X2. It was concluded from the model that when the concentration of Ca2 （X1）， 6BA（X2）and IBA（X3）was 255 mg/L， 0.76 mg/L and 0.16 mg/L respectively， the maximum multiplication rate（Y） was 4.45 which was close to the experiment result. |
| Key words: Acrocarpus fraxinifolius tissue culture proliferation quadratic orthogonal rotation combination design |
