| 摘要: |
| 采用二次正交旋转组合设计对顶果木（Acrocarpus fraxinifolius）增殖培养基进行优化，建立增殖倍数（Y）对培养基中Ca2+浓度（X1）、6 BA浓度（X2）及IBA浓度（X3）的正交回归模型：Y=2.416-0.172X1-0.255X2+0.183X12-0.211X22+0.162X32+0.331X1X2。从模型可知，当Ca2+浓度为255 mg/L，6 BA浓度为0.76 mg/L，IBA浓度为0.16 mg/L时，增殖倍数达最大值4.45。模型预测值与实际验证结果较接近。\ |
| 关键词: 顶果木 组织培养 增殖 二次正交旋转组合设计 |
| DOI： |
| 分类号:S722.3 7 |
| 基金项目:广东省林业科技创新专项“顶果木引种和高效栽培技术研究与示范”（2010KJCX005） |
|
| Study on Optimal Multiplication Medium of Acrocarpus fraxinifolius |
|
XU Weili,XU Chaoxuan,LIN Ronghua and FU Xiaoping
|
|
Zengcheng Forest Farm Guangzhou City，Guangzhou,Zengcheng Forest Farm Guangzhou City，Guangzhou,Guangzhou Forestry Research Institute，Guangzhou
|
| Abstract: |
| The multiplication medium of Acrocarpus fraxinifolius was optimized with quadratic orthogonal rotation combination design. The quadratic orthogonal regression model of multiplication rate（Y） to three factors including concentration of Ca2 （X1）， 6 BA（X2）and IBA（X3） was established as Y=2.420-0.172X1-0.255X2 0.184X12-0.211X22 0.162X32 0.331X1X2. It was concluded from the model that when the concentration of Ca2 （X1）， 6 BA（X2）and IBA（X3）was 255 mg/L， 0.76 mg/L and 0.16 mg/L respectively， the maximum multiplication rate（Y） was 4.45 which was close to the experiment result. |
| Key words: Acrocarpus fraxinifolius tissue culture proliferation quadratic orthogonal rotation combination design |
