摘要: |
采用CTAB法研究不同提取缓冲液对三尖杉DNA 提取质量的影响。结果表明:提取三尖杉DNA的最佳缓冲液所含物质及浓度为100 mmol·L-1TrisHCl pH 8.0+0.14 mol·L-1NaCl+2%CTAB+20 mmol·L-1EDTA +2%β巯基乙醇。采用改进的CTAB法提取了15个三尖杉优树样品的DNA,提取的DNA质量均较高,可以满足分子标记分析的要求。 |
关键词: 三尖杉 CTAB法 DNA提取 |
DOI: |
投稿时间:2013-05-23修订日期:2013-06-05 |
基金项目:福建省林木种苗科技攻关项目(ky0180087) |
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Evaluation of Different Extraction Buffers on the Quality of Cephalotaxus fortunei DNA |
RONG Jun-dong,ZHANG Ying-hui,ZHENG Zhi-lei,CHEN Li-guang,HE Tian-you,ZHENG Yu-shan |
(College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou;Zhangzhou Forestry Administration;College of Forestry,Fujian Agriculture and Forestry University) |
Abstract: |
Different CTAB extraction buffers were studied to evaluate DNA extraction quality of Cephalotaxus fortunei. The results showed that optimal composition of extraction buffers was 100 mmol·L-1TrisHCl pH 8.0 0.14 mol·L-1NaCl 2%CTAB 20 mmol·L-1EDTA 2%βmercaptoethanol. Genomic DNA of 15 samples of C. fortunei plus trees extracted by improved CTAB method could meet molecular marker analysis requirements with high quality. |
Key words: Cephalotaxus fortunei CTAB method Deoxyribonucleic acid (DNA) extraction |