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| 不同提取缓冲液对三尖杉DNA 提取质量的影响 |
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荣俊冬,张迎辉,郑志雷,陈礼光,何天友,郑郁善
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福建农林大学林学院,福建农林大学园林学院,漳州市林业局,福建农林大学林学院,福建农林大学园林学院,福建农林大学林学院
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| 摘要: |
| 采用CTAB法研究不同提取缓冲液对三尖杉DNA 提取质量的影响。结果表明：提取三尖杉DNA的最佳缓冲液所含物质及浓度为100 mmol·L-1TrisHCl pH 8.0+0.14 mol·L-1NaCl+2%CTAB+20 mmol·L-1EDTA +2%β 巯基乙醇。采用改进的CTAB法提取了15个三尖杉优树样品的DNA，提取的DNA质量均较高，可以满足分子标记分析的要求。 |
| 关键词: 三尖杉 CTAB法 DNA提取 |
| DOI： |
| 分类号:S718.43 |
| 基金项目:福建省林木种苗科技攻关项目（ky0180087） |
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| Evaluation of Different Extraction Buffers on the Quality of Cephalotaxus fortunei DNA |
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RONG Jun-dong,ZHANG Ying-hui,ZHENG Zhi-lei,CHEN Li-guang,HE Tian-you and ZHENG Yu-shan
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College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,Zhangzhou Forestry Administration,College of Forestry,Fujian Agriculture and Forestry University,College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,College of Forestry,Fujian Agriculture and Forestry University
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| Abstract: |
| Different CTAB extraction buffers were studied to evaluate DNA extraction quality of Cephalotaxus fortunei. The results showed that optimal composition of extraction buffers was 100 mmol·L-1TrisHCl pH 8.0 0.14 mol·L-1NaCl 2%CTAB 20 mmol·L-1EDTA 2%βmercaptoethanol. Genomic DNA of 15 samples of C. fortunei plus trees extracted by improved CTAB method could meet molecular marker analysis requirements with high quality. |
| Key words: Cephalotaxus fortunei CTAB method Deoxyribonucleic acid (DNA) extraction |
