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马尾松生长和产脂性状在SSR和ESTP标记不同基因型间的变异研究 |
何韵,刘天颐,蔡燕灵,曾令海,黄少伟
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华南农业大学林学院,华南农业大学林学院,广东省林业科学研究院,广东省林业科学研究院,华南农业大学林学院
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摘要: |
对广东省4个试验点内拥有共同母本的马尾松7个全同胞家系和1个自由授粉家系5 a生试验林进行了调查,采用微卫星(SSR)和表达序列标签多态性(ESTP)的分子标记对360个单株进行了检测,筛选得到有多态性的SSR标记4个,ESTP标记15个,位点共21个,其中ESTP标记est8704不同基因型间的产脂量有显著差异,可作为马尾松辅助产脂量选择的候选标记。 |
关键词: 马尾松 生长性状 产脂量 分子标记 标记—性状关联 |
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基金项目:广东省教育部产学研结合项目“高脂马尾松优良品系扦插繁育技术及其产业化”(2007B090400098) |
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Study on the Variation in Growth Traits and Rosin Yield for Different SSR and ESTP Genotypes of Pinus massoniana |
HE Yun,LIU Tianyi,CAI Yanling,ZENG Linghai and HUANG Shaowei
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College of Forestry,South China Agricultural University,Guangdong Forestry Academy,Guangdong Forestry Academy,College of Forestry,South China Agricultural University
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Abstract: |
The study assessed a 5-year old progeny test of Pinus massoniana in four sites in Guangdong Province. One halfsib family and 7 full sib families were surveyed. Totally 360 individual trees were detected with microsatellite (SSR) and expressed sequence tag polymorphism (ESTP) markers. Four polymorphic SSR markers and 15 polymorphic ESTP markers were obtained, with totally 21 loci. Significant difference in turpentine production among genotypes of ESTP marker est8704 was detected, and useful for marker assisted selection of P. massoniana |
Key words: Pinus massoniana growth trait rosin yield molecular marker marker-trait association\ |