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| 核磁共振法测定光皮树优树果实含油量的研究 |
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朱春玲,张良波,李小川,赖旭恩,何祯祥
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上海市林业总站,湖南省林业科学院,广东省林业科学院,龙山林场,南京大学
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| 摘要: |
| 采用核磁共振仪分析21株光皮树优树样品，平均果皮含油量为54.84%，标准差变幅为0.21%～0.92%，变异系数变幅为0.40%～1.52%；种子含油量为10.9%，标准差变幅为0.06%～0.22%，变异系数变幅为0.63%～2.18%，采用核磁共振法测定光皮树果实中的含油量精度较好。与索氏提取法相比，采用核磁共振法分析光皮树果实含油量，样品用量少，测量速度快，且不损伤样品。通过对两种方法的对比测定，相关系数为0.867(果皮)和0.897(种子)。核磁共振法这种快速实用的无损检测技术为生物能源树种光皮树的资源评价、品种改良及育种提供了有效手段。 |
| 关键词: 光皮树〓核磁共振〓含油量 |
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| 基金项目:国家“十一五”科技支撑项目“生物质液体燃料资源光皮树品种选育和丰产栽培技术示范”(2011BAD22B08)；上海市辰山专项“适生能源植物的收集保存和推广应用”(G102409)。 |
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| Study on the Fruit Oil Content of Cornus wilsoniana Plus Trees by \=Nuclear Magnetic Resonance (NMR) Approach |
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Zhu Chunling,Zhang Liangbo,Li Xiaochuan<,Nai Xuen and He Zhenxiang
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Hunan Academy of Forestry,Guangdong Forestry Research Institute,Longshan Forest Farm,Nanjing University
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| Abstract: |
| The oil contents of twenty one samples of Cornus wilsoniana plus trees were analyzed by Nuclear Magnetic Resonance approach. The average oil content of fruit peel was 54.84% with the variation range of stand error 0.21%～0.92%, and that of variation coefficient 0.40%～1.52%; the average oil content of seed was 10.90% with the variation range of stand error 0.06%～0.22%, and that of variation coefficient 0.58%～2.17%. These results illustrated that the accuracy is satisfied in the determination of oil content of C. wilsoniana fruit using HY 10 Nuclear Magnetic Resonance instrument. Compared to the Soxhlet method, Nuclear Magnetic Resonance approach has many advantages such as small quantity of samples needed, rapid measurement, keeping samples intac， etc. Significant correlation between oil contents measured by Nuclear Magnetic Resonance approach and soxhlet method was observed, 0.867 for fruit peel and 0.897 for seed. |
| Key words: Cornus wilsoniana, Nuclear Magnetic Resonance (NMR), oil content |
