| 摘要: |
| 为探索樟树 Camphora officinarum 的组织培养和扦插育苗技术, 解决其繁殖技术难题, 研究以
樟树品种 ‘柠香’ C. officinarum ""Ningxiang"" 的嫩枝为试验材料, 设置不同外植体采集时间、 消毒时间、 生
长调节剂组合及生根环境, 分析各因素对茎段组织培养的影响; 同时, 采用不同激素浓度、 留叶方式、 插
穗直径及基质进行扦插试验, 分析其对插穗生根的影响。 结果表明, 1—4 月为外植体最佳接种期, 无菌
活体获得率高于 50%; E2 (ER+ Ca (NO3 )2·4H2O 150 mg·L
-1
) 为最适基本培养基, 腋芽萌动率达
68. 3%; 诱导丛生芽的最佳激素组合为 6-BA 5. 0 mg·L
-1+NAA 2. 0 mg·L
-1+KT 1. 0 mg·L
-1
, 诱导率
达 75. 8%; 增殖阶段采用 6-BA 3. 0 mg·L
-1+NAA 1. 5 mg·L
-1+KT 0. 2 mg·L
-1
, 继代 35 d 增殖系数达
4. 7, 芽体健壮无玻璃化; 添加 15 mg·L
-1 L-半胱氨酸可显著抑制丛生芽褐化, 褐化率降至 6%; 生根培
养采用 ER+Ca (NO3 )2·4H2O 150 mg·L
-1+IBA 3. 0 mg·L
-1+NAA 2. 0 mg·L
-1+ABT 1. 0 mg·L
-1
, 在
3 000 lx 光照、 25 ℃条件下培养 30 d, 生根率达 87. 2%; 组培生根苗在椰糠与红心土 (1 ∶ 2) 混合基质
中移栽成活率最高, 为 80. 6%; 扦插试验表明, 采用直径 4 mm、 留 2 片完整叶的插穗, 经 100 mg·L
-1
GGR6 处理 2 h 后, 扦插于黄心土 ∶ 泥炭 ∶ 珍珠岩 (3 ∶ 2 ∶ 1) 基质中, 成活率达 97. 53%; 解剖学观察证
实, 樟树扦插生根类型为皮部生根, 不定根起源于维管束形成层薄壁细胞。 研究优化了樟树 ‘柠香’ 组
织培养与扦插育苗的关键技术参数, 初步形成了高效无性繁殖技术体系。 |
| 关键词: 樟树 组培繁育 扦插繁育 生根率 成活率 |
| DOI： |
| 分类号: |
| 基金项目:国家林业和草原局重点研发项目 (GZC [2021] 89), 江西省林业科学院博士启动项目 (2022522701), 广西壮族自治区 林业科技推广示范项目 (2023LYKJ06), 江西省科学技术厅重点研发计划一般项目 (20212BBF63046)。 |
|
| Asexual Propagation System for Camphora officinarum ''Ningxiang'' |
|
Zhu changsan1, ZHANG Xiaoyan2, JIANG Bin3, HU Lifang4, XIE Yangzhi5, SHUAI Xiaopeng3, HUA Xiaoju5, DONG Chen5
|
|
1.Guangxi Forestry Research Institute,Nanning Guangxi;2.Yongfeng County Forestry Development Service Center;3.Guanshan Forest Farm, Yongfeng County;4.Jiangxi Academy of Agricultural Sciences Institute of Agricultural Product Quality and Safety and Standards,;5.Jiangxi Academy of Forestry,Nanchang
|
| Abstract: |
| To explore the tissue culture and cutting seedling techniques of Camphora officinarum and solve
its propagation technical difficulties, this study used the young branches of C. officinarum ""Ningxiang"" as experimental material. The effects of different explant collection times, sterilization time, growth regulator combinations, and rooting environments on tissue culture of stem segments were analyzed. Concurrently, cutting experiments using different hormone concentrations, leaf retention methods, cutting diameters, and substrates were conducted to analyze their effects on cutting rooting. The results indicated that January to April was the optimal explant
collection period, with a sterile live explant obtaining rate of over 50%; E2 medium (ER+Ca (NO3 )2·4H2O
150 mg·L
-1
) was the optimal basal medium, with an axillary bud emergence rate of 68. 3%; the optimal hormone combination for inducing clustered buds was 6-BA 5. 0 mg·L
-1 +NAA 2. 0 mg·L
-1 +KT 1. 0 mg·L
-1
,
with an induction rate of 75. 8%; during the proliferation stage, the combination of 6-BA 3. 0 mg·L
-1+NAA 1. 5
mg·L
-1+KT 0. 2 mg·L
-1 was used, and after 35 days of subculture, the proliferation coefficient reached 4. 7,
with robust buds and no vitrification; adding 15 mg·L
-1
L-cysteine significantly inhibited clustered bud browning, reducing the browning rate to 6%; for rooting culture, the medium ER+Ca (NO3 )2·4H2O 150 mg·L
-1+IBA
3. 0 mg·L
-1+NAA 2. 0 mg·L
-1+ABT 1. 0 mg·L
-1 was used, and under 3 000 lx light and 25 ℃ for 30 days,
the rooting rate reached 87. 2%; the survival rate of tissue-cultured rooted plantlets was highest (80. 6%) when
transplanted into a mixed substrate of coconut chaff and red heart soil (1 ∶ 2). Cutting propagation tests showed
that using cuttings with a diameter of 4 mm and two intact leaves, treated with 100 mg·L
-1 GGR6 for 2 hours,
and then inserted into a substrate of yellow heart soil ∶ peat ∶ perlite (3 ∶ 2 ∶ 1) resulted in a survival rate of
97. 53%. Anatomical observations confirmed that the rooting type of C. officinarum cuttings was bark rooting, and
adventitious roots originated from the parenchyma cells of the vascular cambium. This study optimized the key technical parameters for tissue culture and |
| Key words: Camphora officinarum tissue culture propagation cutting propagation rooting rate survival rate |
