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红锥优树茎段组织培养体系构建
廖焕琴, 徐放, 陈新宇, 朱报著, 张卫华
广东省林业科学研究院
摘要:
为构建红锥 Castanopsis hystrix 优树茎段组织培养体系, 突破红锥优良无性系工厂化育苗瓶颈, 研究在秋季干燥晴朗天气以红锥 22-3 号优树半木质化穗条为外植体, 采用单因素分析, 探讨不同基本培 养基和 6-BA 浓度对增殖效果的影响, 以及不同基本培养基、 不同 IBA 和蔗糖浓度对生根效果的影响。 结果表明, 红锥优树茎段外植体消毒方法为 75%酒精处理 5 s, 无菌水冲洗 1 次, 0. 1%升汞浸泡 5 min, 无菌水冲洗 3 次; 初代诱导培养基为 WPM+2. 0 mg·L -1 6-BA+0. 05 mg·L -1 NAA+15 g·L -1 蔗糖+6. 8 g·L -1 卡拉胶; 适宜的增殖培养基为 WPM+0. 5 mg·L -1 6-BA+30 g·L -1 蔗糖+6. 0 g·L -1 卡拉胶, 增 殖率达 2. 77; 适宜的生根培养基为 1 / 2WPM+2. 0 mg·L -1 IBA+20 g·L -1 蔗糖+6. 0 g·L -1 卡拉胶, 生根 率达 91. 67%; 采用泥炭土 ∶ 蛭石= 1 ∶ 2 (V ∶ V) 基质配方进行移栽, 成活率可达 85%以上。
关键词:  红锥  优树  组织培养
DOI：
分类号:
基金项目:国家自然科学基金青年项目 (32201551)。
Establishment of Tissue Culture System for the Stem Segments of Castanopsis hystrix Dominant Tree
Liao Huanqin, XU Fang, CHEN Xinyu, ZHU Baozhu, ZHANG Weihua
Guangdong Academy of Forestry
Abstract:
To establish a tissue culture system for the stem segments of Castanopsis hystrix, dominant trees and break through the bottleneck of factory seedling cultivation of excellent C. hystrix clones, this study used the semi-lignified cuttings of the C. hystrix dominant tree 22-3 collected under dry and sunny weather in autumn as explants. A single-factor experiment design was employed to investigate the effects of different basic media and 6-BA concentrations on the proliferation efficiency, as well as the effects of different basic media, IBA and sucrose concentrations on the rooting efficiency. The results showed that the optimal disinfection method for the stem segment explants of C. hystrix dominant trees was treatment with 75% alcohol for 5 s, rinsed once with sterile water, followed by immersion in 0. 1% mercuric chloride for 5 min, and than rinsed three times with sterile water. The primary induction medium was WPM+2. 0 mg·L -1 6-BA+0. 05 mg·L -1 NAA+15 g·L -1 sucrose+6. 8 g·L -1 carrageenan. The appropriate proliferation medium was WPM+0. 5 mg·L -1 6-BA+30 g·L -1 sucrose+ 6. 0 g · L -1 carrageenan, with a proliferation rate of 2. 77. The appropriate rooting medium was 1 / 2WPM+ 2. 0 mg·L -1 IBA + 20 g·L -1 sucrose + 6. 0 g·L -1 carrageenan, resulting in a rooting rate of 91. 67%. Using a substrate formula of peat soil ∶ vermiculite = 1 ∶ 2 (V ∶ V) for transplantation, the survival rate could reach over 85%.
Key words:  Castanopsis hystrix  dominant tree  tissue culture