| 摘要: |
| 为便于杜英疫病菌杜英生假隐丛赤壳 Pseudocryphonectria elaeocarpicola 的长期稳定保存和复
苏, 研究采用 5 种保存方法 (石蜡 4 ℃、 无菌水室温、 甘油-80 ℃、 甘油-20 ℃ (24 h) + -80 ℃和孢子
悬浮液-80 ℃) 处理分离自尖叶杜英 Elaeocarpus rugosus 的两株病原菌 SDY 和 LDY, 保存时间为 3 ~ 12
个月。 每月菌株取样复苏后, 通过十字交叉法测定菌落直径, 并计算其生长速率。 保存 12 个月后, 对菌
株进行接种试验, 计算病斑面积, 评估其致病性。 结果显示, 不同保存条件下病原菌的生长速率差异显著
(P<0. 05), 石蜡 4 ℃保存法处理的病原菌生长速率随着保存时间延长逐渐减小, 直至停止, 而其他方法
保存的病原菌生长速率表现为先下降后回升趋于稳定; 菌株 SDY 和 LDY 不同保存时间的生长速率均存在
显著差异 (P<0. 05)。 致病性结果表明, 菌株采用无菌水室温、 甘油-80 ℃和甘油-20 ℃ (24 h) +-80 ℃保
存 12 个月后仍具有致病性, 且和新分离病原菌相比, 致病面积差异不显著 (P>0. 05), 是杜英疫病菌较适宜
的保存方法。 其中, 无菌水室温保存操作最简便, 需通过无菌操作, 降低霉菌和细菌污染的风险。 |
| 关键词: 杜英疫病 保存方法 杜英生假隐丛赤壳 |
| DOI： |
| 分类号: |
| 基金项目:广东省基础与应用基础研究基金 (2023A1515011968)。 |
|
| Selection of Optimal Preservation Methods for the Pathogen Causing Stem Blight of Elaeocarpus |
|
zhaoyi, huanghuayi, zhaodanyang, daijian, gaochenglong, hulili, chenshiying, GUAN Lili
|
|
Guangdong Academy of Forestry
|
| Abstract: |
| For the long-term stable preservation and revival of Pseudocryphonectria elaeocarpicola, five
preservation methods were used to treat two pathogen strains SDY and LDY isolated from Elaeocarpus rugosus
for 3 to 12 months. The methods were paraffin at 4 ℃, sterile water at room temperature, glycerol preserved at
-80 ℃, glycerol at -20 ℃ for 24 h then at -80 ℃, and spore suspension at -80 ℃. Each month, strains
were sampled and revived. The colony diameter was measured by the cross-streak method, and the growth rate
was calculated. After 12 months of preservation, inoculation tests were conducted on the strains. The lesion
area was calculated, and the pathogenicity was evaluated. The results showed that the growth rates of the pathogen under different preservation conditions had significant differences (P<0. 05). The growth rates of the
pathogen preserved with the paraffin at 4 ℃ method gradually decreased with extended preservation time until it
stopped, while the growth rates of pathogens preserved by the other methods showed an initial decrease followed
by a rebound and stabilization. The growth rates of strains SDY and LDY at different preservation times all had
significant differences (P<0. 05). The pathogenicity results indicated that strains preserved by sterile water at
room temperature, glycerol at -80 ℃, and glycerol at -20 ℃ for 24 h then at -80 ℃ for 12 months still possessed pathogenicity. Compared with the newly isolated pathogen, the difference in lesion area was not significant (P>0. 05). These are more suitable methods for P. elaeocarpicola. Among them, the method of preservation in sterile water at room temperature has the simplest procedure. Aseptic technique is required to reduce the
risk of mold and bacterial contamination. |
| Key words: stem blight of Elaeocarpus preservation methods Pseudocryphonectria elaeocarpicola |
