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基于 SLAF-seq 技术的湿加松遗传多样性 和遗传结构分析
王哲1, 邓乐平1, 龙永彬1, 谢 威2, 曾 明1, 欧阳曦1, 郭文冰1, 李义良1
1.广东省林业科学研究院;2.台山市红岭种子园
摘要:
为了分析湿加松 Pinus elliottii × P. caribaea 育种群体的遗传多样性和遗传结构, 并构建核心 种质, 辅助湿加松高世代育种, 研究以台山市红岭种子园内湿加松初级种子园的 50 个无性系和湿加松育 种园的 66 个优良个体为材料, 通过 SLAF-seq 技术开发 126 365 个高质量 SNP 标记。 遗传多样性分析结 果显示, 湿加松育种群体期望杂合度 (He)、 观测杂合度 (Ho) 分别为 0. 280 6、 0. 210 3, Nei 多样性指 数 (H)、 多态性信息含量 (PIC) 和香浓-维纳指数 (I) 分别为 0. 281 9、 0. 232 3 和 0. 441 9。 系统发育 树将所有样本分为 3 个大分支, 群体结构最佳类群数量 K= 6, 其中类群 Q4 包含的种子园样本最少。 评选 出了 23 份材料构建核心种质, 遗传多样性覆盖度超过 99%, 分别来自 6 个类群, 其中只有 7 个来自初级 种子园。 该研究表明湿加松育种群体具有中等的遗传多样性和多源的遗传结构, 揭示了湿加松初级种子 园相较于整个育种群体在遗传结构方面存在一定的缺陷。
关键词:  湿加松  SLAF-seq  遗传多样性  遗传结构
DOI：
分类号:
基金项目:国家自然科学基金青年科学基金项目 (32101548)。
Analysis of Genetic Diversity and Genetic Structure of the Population of Pinus elliottii × P. caribaea Based on SLAF-seq Technology
wang zhe1, DENG Leping1, long yongbin1, XIE Wei2, ZENG Ming1, OUYANG Xi1, GUO Wenbing1, LI Yiliang1
1.Guangdong Academy of Forestry;2.Hongling Seed Orchard
Abstract:
This study aims to analyze the genetic diversity and structure of Pinus elliottii × P. caribaea breeding population and to establish a core germplasm to assist in high-generation breeding. This study used 50 clones from the primary seed orchard and 66 superior individuals from the breeding orchard of P. elliottii × P. caribaea in Hongling Seed Orchard, Taishan city as materials. A total of 126 365 high-quality SNP markers were developed through SLAF-seq technology. Genetic diversity analysis revealed that the expected heterozygosity (He) and observed heterozygosity (Ho) were 0. 280 6 and 0. 210 3, respectively. The Nei′s diversity index (H), polymorphism information content (PIC), and Shannon-Wiener index (I) were 0. 281 9, 0. 232 3, and 0. 441 9, respectively. The phylogenetic tree divided all samples into three major branches. The optimal number of groups in the population structure was K= 6, and group Q4 contained the fewest samples from the seed orchard. Twenty-three materials were selected to construct the core collection, with a genetic diversity coverage of over 99%. These materials were from six groups, and only seven of them were from the primary seed orchard. This study indicates that the P. elliottii × P. caribaea breeding population has moderate genetic diversity and a multi-source genetic structure, revealing certain deficiencies in the genetic structure of the primary seed orchard of P. elliottii × P. caribaea.
Key words:  Pinus elliottii × P. caribaea  SLAF-seq  genetic diversity  genetic structure