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广东含笑无性系苗期叶片功能性状和生长综合评价
刘俊涛, 张卫华, 朱报著, 徐放, 白青松, 廖焕琴, 陈新宇, 郭镜赟
广东省林业科学研究院
摘要:
为探究广东含笑 Michelia guangdongensis 不同无性系苗木生长和叶结构功能性状的变异规律, 以 38 份广东含笑无性系苗木为材料, 测定苗高、 地径和叶结构功能性状 (叶片 SPAD、 叶面积、 叶片厚 度、 叶片体积、 比叶面积、 叶组织密度、 叶片干物质量、 叶片相对含水率、 叶片背部颜色和绒毛量), 分 析苗木生长和叶片功能性状的变异程度及相关性, 对各指标进行初步选择评价。 结果表明, 不同无性系 的苗高、 地径、 SPAD、 叶面积等 9 个性状差异极显著 (P<0. 01), 叶组织密度差异不显著, 各性状变异 系数的变化范围在 5. 14% ~20. 94%之间。 除了叶组织密度、 地径, 其余性状的重复力均高于 0. 5。 相关分 析显示, 叶面积、 比叶面积、 叶片体积与叶片干物质量、 叶组织密度、 叶片相对含水率呈极显著正或负 相关 (P<0. 01)。 比叶面积与叶面积呈极显著正相关 (P<0. 01), 而与叶片厚度、 叶干物质含量、 叶组织 密度、 叶片相对含水率均呈极显著负相关 (P<0. 01)。 采用熵权-TOPSIS 法对广东含笑不同无性系苗木 生长和叶结构功能性状进行选择评价, 排名前 5 的广东含笑无性系为 CG3、 1、 5、 8804、 8999, Ci 值均 在 0. 6 以上, 5 个无性系的苗高、 叶面积比无性系群体平均值高 4. 68%、 24. 25%, 遗传增益分别达到 3. 84%和 22. 12%, 而且叶片背部的呈色效果较好, 且绒毛较多和颜色较深, 观赏效果较优。
关键词:  广东含笑  无性系  叶片功能性状  相关关系  熵权-TOPSIS 法
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基金项目:国家林业和草原局科技发展中心生物安全与遗传资源管理专项经费项目 (KJZXSA202311), 广东省林业局项目 (粤财预 [2023] 6 号)
Comprehensive Evaluation of Functional Traits and Growth of Seedling Leaf Blades of Lines of Michelia guangdongensis
LIU Juntao, ZHANG Weihua, ZHU Baozhu, XU Fang, BAI Qingsong, LIAO Huanqin, CHEN Xinyu, GUO Jingyun
Guangdong Forestry Research Institute
Abstract:
To investigate the variation patterns of growth and leaf structural and functional traits of different Michelia guangdongensis line seedlings, a total of 38 M. guangdongensis line seedlings were used as materials, and the seedling height, ground diameter, and 12 traits related to leaf structure and functional traits ( leaf SPAD, leaf area, leaf thickness, leaf volume, specific leaf area, leaf tissue density, leaf dry mass, leaf rela-tive water content, leaf dorsal color, and leaf pubescence amount) were 12 related traits. The variation degree and correlation of seedling growth and leaf structure and function traits were analyzed, and a comprehensive evaluation was carried out. The results showed significant differences in seedling height, ground diameter, SPAD, leaf area, and other 9 traits among different clones (P<0. 01), but there was no significant difference in leaf tissue density. The coefficients of variation of each trait ranged from 5. 14% to 20. 94%. Except for leaf tissue density and ground diameter, the repeatability of other traits was higher than 0. 5. According to the correlation analysis, leaf area, specific leaf area, and leaf volume were significantly positively or negatively correlated with leaf dry matter mass, leaf tissue density, and leaf relative water content (P<0. 01). Specific leaf area was significantly positively correlated with leaf area (P<0. 01), while it was significantly negatively correlated with leaf thickness, leaf dry matter content, leaf tissue density, and leaf relative water content (P < 0. 01). The entropy-weighted TOPSIS method was used to comprehensively evaluate the growth and leaf structure functional traits of seedlings of different M. guangdongensis line, and the Ci values of the selected M. guangdongensis line CG3, 1, 5, 8804, and 8999 were all above 0. 6. The seedling height and leaf area of the five clones were 4. 68% and 24. 25% higher than the average value of the line population, and the genetic gains were 3. 84% and 22. 12%, respectively. Its leaf dorsal coloration was better, more velvety and darker, and the ornamental effect was better.
Key words:  Michelia guangdongensis  different asexual lines  Leaf functional traits  correlation  entropy-weighted tosis method