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广佛手响应柑橘锈螨为害的转录组分析
雷梦英1, 张智2, 林培华2, 冼国雄2, 李落叶1, 郭健玲1, 胡益波2, 刘 超3
1.广东生态工程职业学院;2.德庆县农业技术推广中心;3.广东生态工程职业学院林业工程学院
摘要:
为探究广佛手 Citrus medica ""Fingered"" 响应柑橘锈螨 Phyllocoptruta oleivora 为害的分子机制, 研究采用 Illumina 测序技术对柑橘锈螨为害的广佛手果皮进行了转录组测序和分析。 试验共获得 1 005 个 差异表达基因, 其中 271 个基因表达上调, 734 个基因表达下调。 GO 功能富集和 KEGG 代谢通路分析发 现, 差异表达基因显著富集在代谢途径、 次生代谢产物的生物合成、 植物-病原体相互作用等通路。 其 中, 植物-病原体互作通路中有 42 个基因的表达显著变化, 大部分生长素早期响应蛋白基因和生长素响 应因子被不同程度激活。 81 个转录因子在柑橘锈螨为害后差异表达, 下调基因多于上调基因。 利用实时 荧光定量 PCR 验证了随机挑选的差异基因在柑橘锈螨为害的广佛手和正常广佛手果实的外果皮中的相对 表达量, 与转录组测序结果的变化趋势一致。
关键词:  广佛手  柑橘锈螨  转录组测序  差异表达基因
DOI：
分类号:
基金项目:广州市科技计划项目 (2024E04J1247), 广东省普通高校创新团队项目 (2022KCXTD050)。
Transcriptome Analysis of Citrus medica ""Fingered"" Response to Phyllocoptruta oleivora Infestation
LEI Mengying1, ZHANG Zhi2, LIN Peihua2, XIAN Guoxiong2, LI Luoye1, GUO Jianling1, HU Yibo2, LIU Chao3
1.Guangdong Eco-Engineering Polytechnic,Guangzhou;2.Deqing Agricultural Technology Extension Center,Zhaoqing;3.Eco-Engineering Polytechnic
Abstract:
To explore the molecular response mechanism of Citrus medica "" Fingered "" to Phyllocoptruta oleivora infestation, the C. medica ""Fingered"" pericarps, inoculated either with or without P. oleivora, were collected for transcriptional sequencing and analysis based on Illumina sequencing platform. In total, the infection by P. oleivora caused the 1 005 differentially expressed genes (DEGs) in C. medica ""Fingered"" fruits, including 271 up-regulated DEGs and 734 down-regulated DEGs. The analyses of GO functional enrichment and KEGG metabolic pathways revealed that these DEGs were significantly enriched in the pathways of metabolism, secondary metabolites biosynthesis, plant-pathogen interaction, among others. Of these, the expression of 42 genes in the plant-pathogen interaction pathway was significantly altered, most genes of early auxin response proteins and auxin response factors were up -regulated at different degrees. Furthermore, a total of 81 transcription factors (TFs) were differentially expressed with significance, tending more towards being down-regulated. The relative expression levels of randomly selected differentially expressed genes in the exocarp of C. medica ""Fingered"" fruits infested by P. oleivora and normal fruits were validated using real-time quantitative PCR, which were consistent with the trends observed in the transcriptome sequencing results.
Key words:  Citrus medica ""Fingered ""  Phyllocoptruta oleivora  transcriptional sequencing  differentially expressed genes