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杜英疫病病原菌生物学特性∗
官莉莉,黄华毅,戴 坚,高成龙,赵奕,王洋文
广东省林业科学研究院/广东省森林培育与保护利用重点实验室
摘要:
为明确杜英疫病病原菌杜英生假隐丛赤壳 Pseudocryphonectria elaeocarpicola 的生物学特性及 其对山杜英 Elaeocarpus sylvestris 的致病性, 采用生长速率法测定了杜英疫病病原菌最适生长碳源、 氮源、 温度、 pH 值等生物学特性, 并通过烫伤接种的方法, 以菌饼接种山杜英盆栽苗木探究其致病性。 研究结 果表明, 杜英疫病病原菌最适生长碳源为可溶性淀粉、 乳糖和纤维素粉, 氮源为蛋白胨和酵母浸粉, pH 值为 4, 生长温度为 30 ℃, 其致死温度为 55 ℃, 光照条件不影响其菌丝生长。 接种实验结果表明该病原 菌对山杜英具有致病性。
关键词:  杜英疫病  杜英生假隐丛赤壳  生物学特性  山杜英  致病性
DOI:
分类号:
基金项目:广东省林业科技创新项目 (2020KJCX004, 2023KJCX022)。
Biological Characteristics of the Pathogen for Stem Blight of Elaeocarpus
GUAN Li-li1,2, HUANG Hua-yi1,2, DAI Jian1,2, GAO Chen-long1,2, ZHAO Yi1,2, WANG Yang-wen1,2
1.Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou;2.China
Abstract:
To identify the biological characteristics of Pseudocryphonectria elaeocarpicola, the pathogen for stem blight of Elaeocarpus, and to explain its pathogenicity to Elaeocarpus sylvestris, the biological characteristics such as carbon source, nitrogen source, temperature, pH and light exposure of the pathogen were measured by growth rate method. The pathogenicity of the pathogen was studied by inoculating the potted seedlings by scalding inoculation. The results showed that the optimum carbon source for the growth of the pathogen was soluble starch, lactose and cellulose, the optimum nitrogen source was peptone and yeast extract, the optimal pH value was 4, the optimal growth temperature was 30 °C, the lethal temperature was 55 °C and the light exposures did not significantly affect the fungal growth. The inoculation experiment results showed that the pathogen was pathogenic to Elaeocarpus sylvestris.
Key words:  stem blight of Elaeocarpus  Pseudocryphonectria elaeocarpicola  biological characteristics  Elaeocarpus sylvestris  pathogenicity