| 摘要: |
| 为筛选出抗寒性较强的山茶 Camellia japonica 品种, 提高栽培效率, 选取信阳地区 6 个山茶品
种为试验材料, 分别为 ‘龙火珠’ C. japonica ′Long-huo-zhu′、 ‘贝拉玫瑰’ C. japonica ′Bei-la-meigui′、 ‘赤丹’ C. japonica ′Chi-dan′、 ‘耐冬’ C. japonica ′Nai-dong′、 ‘七星球’ C. japonica ′Qi-xingqiu′以及 ‘帕克斯’ C. japonica ′Pa-ke-si′, 在 3 个温度下 (-3、 0、 3 ℃) 进行采样, 测定叶片的抗氧化
酶活性、 渗透调节物质以及丙二醛 (MDA) 含量, 运用主成分分析法和隶属函数法对山茶抗寒性进行综
合评价。 结果表明, 与 0 ℃和 3 ℃条件相比, 低温 (-3 ℃) 显著提高了 ‘龙火珠’ ‘贝拉玫瑰’ 和 ‘七
星球’ 的超氧化物歧化酶活性 (P<0. 05), 显著增加了 ‘七星球’ 和 ‘帕克斯’ 的可溶性蛋白质含量,
降低了 ‘龙火珠’ ‘贝拉玫瑰’ 和 ‘耐冬’ 的 MDA 含量 (P<0. 05)。 主成分分析显示, 山茶不同品种的
耐寒性主要由保护酶系统和渗透调节物质共同作用决定。 利用隶属函数法对 6 个山茶品种进行综合分析得
到耐寒性强弱顺序依次为 ‘贝拉玫瑰’ > ‘龙火珠’ > ‘赤丹’ > ‘七星球’ > ‘耐冬’ > ‘帕克斯’。 |
| 关键词: 山茶 抗寒性 隶属函数法 生理指标 低温胁迫 |
| DOI： |
| 分类号: |
| 基金项目:国家林业与草原局科技发展中心生物安全与遗传资源管理项目 (SAKJZX2019044)。 |
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| Evaluation of Cold Resistance of Different Camellia japonica Clones in Central China—A Case Study of Xinyang City |
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Wang Xiao1, Peng Shuang1, Duan Xiaohui1, Yan Shixian1, Guan Zhuizhui2, Ma Tianxiao2
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1.Forest Farm in Heiyan Town, Xixia County;2.Xinyang Agriculture and Forestry University
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| Abstract: |
| To screen out Camellia japonica varieties with strong cold resistance and improve cultivation efficiency. Six clones of C. japonica from Xinyang city, including C. japonica ′Long-huo-zhu′, C. japonica ′Bei
-la-mei-gui′, C. japonica ′Chi-dan′, C. japonica ′Nai-dong′, C. japonica ′Qi-xing-qiu′, and C. japonica ′
Pa-ke -si′ were chosen as experimental materials. The samples were collected at three different temperatures
( -3, 0, and 3 ℃), in order to assess various physiological and biochemical indicators, including the levels of malondialdehyde ( MDA) in the leaves, antioxidant enzyme activity, and osmotic regulating chemicals. A
thorough assessment of C. japonica’ s resistance to cold was conducted using the principal component analysis
and membership function method. The results showed that in comparison to 0 and 3 ℃, a low temperature of -
3 ℃ significantly increased the superoxide dismutase ( SOD) activity of the clones C. japonica ′Long -huo -
zhu′, C. japonica ′Bei-la-mei-gui′, and C. japonica ′Qi-xing-qiu′. It also significantly increased the soluble
protein contents of the clones C. japonica ′Pa-ke-si′ and C. japonica ′Qi-xing-qiu′, and decreased the MDA
contents of the clones C. japonica ′Long-huo-zhu′, C. japonica ′Bei-la-mei-gui′, and C. japonica ′Nai -
dong′. Principal component analysis revealed that the cold tolerance of several C. japonica clones was mostly
due to the combined activity of protective enzyme systems and osmotic adjustment chemicals. The cold resistance of the six C. japonica clones was thoroughly analyzed using the membership function approach, and the order of cold resistance was C. japonica ′Bei-la-mei-gui′>C. japonica ′Long-huo-zhu′>C. japonica ′Chi-dan′>
C. japonica ′Qi-xing-qiu′>C. japonica ′Nai-dong′>C. japonica ′Pa-ke-si′. |
| Key words: Camellia japonica cold resistance subordination function method physiological index low-temperature stress |
