| 摘要: |
| 为明确无瓣海桑炭疽病的病原菌种类, 为该病害的有效防控提供基本依据, 采集无瓣海桑
Sonneratia apetala 受害叶片, 通过组织分离法获得菌株, 利用柯赫氏法则确定病原菌; 利用形态学及分子
生物学分析方法明确病原菌的分类地位, 同时开展生物学特性分析。 通过接种试验确定, 从病组织中分
离获得菌株 WBHS-YK-1 为无瓣海桑炭疽病的病原菌。 该菌在 PDA 上培养 7 d, 菌落圆形, 气生菌丝白
色, 培养基底部有青黑色色素沉淀, 同时产生黄色的分生孢子堆; 分生孢子呈长椭圆, 无色, 无隔膜,
孢子大小为 (15~19) μm× (6~8) μm。 基于 ITS 序列构建的系统发育树显示: 该菌株与菌种 Colletotrichum fructicola 聚在同一分支, 支持率高达 98%, 表明该菌与 C. fructicola 具有较高的亲缘关系。 结合形态
学特征, 最终确定菌株 WBHS-YK-1 为果生炭疽菌菌 C. fructicola。 该菌能最佳碳源为葡萄糖, 最佳氮源为
蛋白胨; 最适培养温度为 30 ℃, 最适 pH 为 7~8。 |
| 关键词: 无瓣海桑 叶斑病 鉴定 果生刺盘孢 生物学特性 |
| DOI： |
| 分类号: |
| 基金项目:广东省林业科技创新项目 (2023KJCX020)。 |
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| Identification and Biological Characterization of Sonneratia apetala Buchanan-Hamilton leaf spot Pathogen |
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xiao haiyan1, feng jin yan2, lu jian kan2, zhang liang jun2, zheng shi jing2, xu jin zhu2, tian long yan2, XIAO Lina2
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1.韶关市国有林场事务中心;2.Guang Dong Academy of Forestry
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| Abstract: |
| The pathogen was identified to provide a theoretical basis for effective prevention and control of
anthracnose in Sonneratia apetala. Microbes were isolated and purified from the S. apetala leaves showing
anthracnose symptoms. Using Koch′s method, the pathogenicity of the isolates was tested and verified. Thetaxonomy of pathogens was determined by morphological observations and molecular technology. Strain WBHSYK-1 was isolated from the diseased tissue and proved to be the pathogen that caused the anthracnose symptoms in S. apetala leaves. The colonies WBHS-YK-1 were white with cyan-black precipitates round after 7 d on
PDA. The conidia were elliptical and 15-19 μm×6-8 μm in size. ITS fragments were used to construct the
phylogenetic tree that showed WBHS-YK-1 to cluster with Colletotrichum fructicola in the same branch, the
support rate was as high as 98%. The best carbon source for this bacterium is glucose, and the best nitrogen
source is peptone; The optimal cultivation temperature is 30 ℃ and the optimal pH is 7-8. |
| Key words: anthracnose of Sonneratia apetala Colletotrichum fructicola biological characteristics |
