| 摘要: |
| 为了明确高压注射 2%甲维盐乳油对林间松材线虫病的防治效果, 研究分析了注射甲维盐后林
间马尾松 Pinus massoniana 体内残留量的时间动态变化及甲维盐对树体内松材线虫 Bursaphelenchus xylophilus 种群的抑制作用, 并研究了注射甲维盐对寄主马尾松活性氧 (Reactive oxygen species, ROS) 水平
和功能微生物的影响, 从不同层面综合评价了甲维盐对林间松材线虫病的直接和间接防治效果。 结果表
明, 甲维盐注干 18 个月后, 试验区马尾松植株保存率超过 98%。 注射甲维盐 1 a 后, 马尾松树体内树干
和枝条均可检测到残留, 在树体上部、 中部及冠层枝条呈下降趋势, 树体下部在试验期间呈上升趋势且
马尾松体内残留量均高于 LC95 (0. 05 μg·g
-1
); 注射甲维盐后, 室内外马尾松植株体内松材线虫数量较
对照显著下降 (P<0. 05), 室内马尾松幼苗体内松材线虫数量下降 61. 02%, 林间松材线虫高携带单株
(≥5 000) 和低携带单株 ( <5 000) 体内松材线虫携带量分别减少 93. 01%和 71. 09%; 注射甲维盐能显
著提升马尾松植株体内 ROS 水平 (P<0. 05), 室内接种松材线虫的马尾松幼苗、 林间松材线虫高携带量
和低携带量的马尾松个体的 ROS 水平分别为对照的 7. 96、 1. 90 和 6. 59 倍; 马尾松中抗性相关微生物
Penicillium 的丰度均显著提高 (P<0. 05), 松材线虫携带量高和低的马尾松体内较对照分别提升 19. 12%
和 3. 15%。 研究结果表明了树干注射甲维盐 2%乳油可显著林间降低马尾松植株体内的松材线虫数量, 并
提升寄主马尾松的活性氧水平及 Penicillium 属丰度。 |
| 关键词: 松材线虫病 马尾松 2%甲维盐乳油 残留量 ROS 防治效果 |
| DOI： |
| 分类号: |
| 基金项目:福建省林业科技项目 (ZMGG-0705) |
|
| Comprehensive Evaluation About Control Effect of Trunk Injections of Emaectin Benzoate on Pine Wilt Disease in the Field |
|
Song Gaofei1, Zhang Biaoqiang2, Tong Hao3, Lan Wenfeng4, Zhang Zhihao2, Tang Chensheng5, Cai Shouping5
|
|
1.Syngenta (China) Investment Co., Ltd;2.Guanzhuang State Forestry Farm;3.Shuinan State Forestry Farm;4.Wuyi State Forestry Farm;5.Fujian Academy of Forestry/ The Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China
|
| Abstract: |
| To clarify the effect of high-pressure injection of 2% Emamectin benzoate (EB) on the control
of pine wilt disease (PWD) in the forest, this study analyzed the temporal dynamics of residues in Pinus massoniana and the inhibitory effect of EB on Bursaphelenchus xylophilus populations in the trees, and also studied the
impact of EB on the levels of reactive oxygen species ( ROS) and functional microorganisms in the host
P. massoniana. From different perspectives, the study comprehensively evaluated the direct and indirect control
effects of EB on PWD in forests. The results showed that the preservation rate of P. massoniana plants in the experimental area was over 98% after 18 months of EB injection. One year after the injection of EB, residues
could be detected in the trunks and branches of P. massoniana trees, with a decreasing trend in the upper, middle parts and canopy branches, and an increasing trend in the lower part of the tree during the test period. The
residues levels in P. massoniana trees were higher than LC95 (0. 05 μg·g
-1
). After the injection of EB, the
number of B. xylophilus in P. massoniana plants indoors and outdoors decreased significantly compared to the
control (P<0. 05). The number of B. xylophilus in indoor P. massoniana seedlings decreased by 61. 02%, and
the number of B. xylophilus in P. massoniana with high (≥5 000 nematodes) and low ( <5 000 nematodes)
burdens of B. xylophilus in the forest decreased by 93. 01% and 71. 09%, respectively. The injection of EB significantly increased the ROS level in P. massoniana plants ( P < 0. 05 ), and the ROS levels in indoor
P. massoniana seedlings inoculated with B. xylophilus, and in individual P. massoniana trees with high and low
burdens of B. xylophilus in forest stands, were 7. 96, 1. 90, and 6. 59 times that of the control, respectively.
The abundance of Penicillium, a resistance-associated microorganism, was significantly higher (P<0. 05) in
both P. massoniana seedlings and trees, and increased by 19. 12% and 3. 15%, respectively, in trees with with
high and low burdens of B. xylophilus compared to the control. The research results indicate that stem injection
of 2% EB can significantly reduce the number of B. xylophilus in P. massoniana trees and increase ROS levels
and Penicillium abundance in the host. |
| Key words: pine wilt disease Pinus massoniana 2% Emamectin benzoate residue ROS control efficiency |
