| 摘要: |
| 为探讨盐、 干旱胁迫对中亚沙棘 Hippophae rhamnoides subsp. turkestanica 种子萌发和幼苗生长
的影响, 该研究采用等渗 NaCl 和 PEG6000 溶液模拟盐和干旱胁迫, 以清水处理为对照组 (CK), 设置
-0. 2、 -0. 4、 -0. 6、 -0. 8 和-1. 0 MPa 共 5 个渗透势水平, 每日观察并记录中亚沙棘种子萌发和幼苗生
长状况。 结果表明, 中亚沙棘种子最终萌发率随 PEG 溶液渗透势降低而降低, 而 NaCl 溶液对其没有显著
影响; NaCl 和 PEG 胁迫下中亚沙棘的最终萌发率分别为 96. 7% ~ 100%和 0 ~ 97. 5%, NaCl 对其萌发影响
不显著, 而 PEG 影响显著 (P<0. 05); PEG 处理组复水后萌发率达 100%, 表明干旱胁迫可导致种子休
眠, 但种子的活力仍能保持。 NaCl 和 PEG 胁迫延后了其初始萌发时间并延长萌发周期, 且 PEG 胁迫的抑
制作用较 NaCl 胁迫剧烈。 通过对中亚沙棘幼苗的鲜质量和苗高统计分析, 发现 NaCl 处理组的中亚沙棘
幼苗鲜质量先升高后降低, PEG 处理组鲜质量明显降低; NaCl 和 PEG 对幼苗苗高有抑制作用, 处理组苗
高均低于 CK, 且 NaCl 胁迫主要表现在根伸长生长受到抑制, PEG 胁迫组根短且弯曲。 PEG 对中亚沙棘
种子萌发和幼苗生长的抑制程度强于等渗 NaCl, 说明在盐和干旱胁迫条件下, 渗透胁迫是影响其萌发的
主要因素, 中亚沙棘对干旱胁迫较盐胁迫更敏感, 同时对盐胁迫具有一定耐受性。 |
| 关键词: 盐胁迫 PEG 胁迫 复水 种子萌发 幼苗生长 中亚沙棘 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金 (31660048) |
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| Effect of Iso-osmotic NaCl and PEG6000 Stress on Seed Germination and Seedling Growth of Hippophae rhamnoides subsp. turkestanica |
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ZHUANG Zhong,Zhang Ling,QIU Aijun,LIU Yanping,ZHOU Mei
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1.Tarim University;2.College of Chemical and Technology, Tianshui Normal University
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| Abstract: |
| To investigate the effects of salt and drought stress on seed germination and seedling growth of
Hippophae rhamnoides subsp. turkestanica, iso-osmotic NaCl and PEG6000 solutions were used to simulate salt and drought stress, respectively, and water treatment was used as the control group (CK). Five osmotic potential levels of -0. 2, -0. 4, -0. 6, -0. 8, and -1. 0 MPa were set up, and the seed germination and seedling growth of H. rhamnoides subsp. turkestanica was observed and recorded daily. The results showed that the
seed final germination rate decreased with the decrease in the osmotic potential of PEG solution, while NaCl solution had no significant effect. The final germination rates of H. rhamnoides subsp. turkestanica under NaCl and
PEG stress were 96. 7%-100% and 0-97. 5%, respectively. NaCl had no significant effect on its germination,
while PEG had a significant effect (P<0. 05). The germination rate of the PEG treatment group was 100% after
rehydration, indicating that drought stress could lead to seed dormancy, but seed vigor could still be maintained. NaCl and PEG stress delayed the initial germination time and prolonged the germination cycle, with the
inhibitory effect of iso-osmotic PEG stress being stronger than that of NaCl stress. Through the statistical analysis of the fresh weight and seedling height of seabuckthorn seedlings, it was found that the seedling fresh weight
in NaCl treatment group increased first and then decreased, and the fresh weight of iso-osmotic PEG treatment
group decreased significantly. NaCl and PEG inhibited the seedling height, and the seedling height of the treatment group was lower than that of CK, and NaCl stress was mainly manifested in the inhibition of root elongation growth, and the root of PEG stress group was short and curved. The inhibition of iso-osmotic PEG on seed
germination and seedling growth of H. rhamnoides subsp. turkestanica was stronger than that of isotonic NaCl,
indicating that osmotic stress was the main factor affecting its germination under salt and drought stress conditions. H. rhamnoides subsp. turkestanica was more sensitive to drought stress than salt stress, and had a certain
tolerance to salt stress. |
| Key words: salt stress PEG stress rewatering seed germination seedling growth Hippophae rhamnoides subsp. turkestanica |
