| 摘要: |
| 为丰富惠州市特有极小种群植物博罗红豆 Ormosia boluoensis 的叶绿体基因组资料, 进一步为
其种质资源鉴定, 分子标记开发提供参考依据, 文章基于高通量测序方法完成了博罗红豆不同分布点的
叶绿体基因组测序, 并运用生物信息学和系统基因组学方法分析其结构、 基因构成的特征。 结果表明:
(1) 4 个采样点博罗红豆的基因组长度分别为 174 713、 174 694、 174 695、 174 861 bp, 基因组总 GC 含
量分别为 36. 22%、 35. 76%、 36. 42%、 36. 07%, 都具有典型的环状四分体结构; (2) 博罗红豆叶绿体基
因组包含有 142 个基因, 包括 97 个蛋白质编码基因 (PCG), 8 个核糖体 RNA 基因 (rRNA), 37 个转运
RNA 基因 (tRNA); (3) 博罗红豆叶绿体基因组中共检测到 28 225 个密码子, 这些密码子中编码亮氨酸
(Leu)、 丝氨酸 (Ser) 和精氨酸 (Arg) 的种类最多且均达到 6 种, 使用频率最多的是编码异亮氨酸
(Ile) 的 AUU (1 241 个) 密码子; (4) 进一步对其简单重复序列 (SSR) 及核苷酸多样性分析结果表
明, 博罗红豆叶绿体基因组具有较高的单核苷酸重复类型 SSR, 并在 LSC 区域检测到 4 个核苷酸多态性
变异热点区域。 |
| 关键词: 叶绿体基因组 测序 特征 博罗红豆 |
| DOI： |
| 分类号: |
| 基金项目:惠州市科技计划项目(2022CQ010011) |
|
| Sequencing and Characterization of Chloroplast Genome of Ormosia boluoensis |
|
liudehao, chenzhitao, zhouxiaofeng, wanggaofeng, shuxiazhu, zhongxiaohua, wangshaodong, lishiqi
|
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(Huizhou Institute of Forestry（Huizhou Botanical Garden Management Service Center）
|
| Abstract: |
| The Ormosia boluoensis is one of the second-class protected plants in China. The aim of this
study is to enrich the data of chloroplast genome, and provide a reference for further research on exploitation of
the germplasm resources and molecular marker development. This study sequenced the chloroplast genome of
O. boluoensis using high-throughput sequencing methods, analyzed its structural and genetic characteristics using
bioinformatics methods, and explored its phylogenetic relationships. The results showed that: (1) The genome
length ratios of NKS01, XTS01, XTS02, and XTS03 were 174 713 bp, 174 694 bp, 174 695 bp, 174 861 bp,
and the total GC content of the genome was 36. 22%, 35. 76%, 36. 42%, and 36. 07%, respectively, in four
different populations of Borneo beans, exhibiting a typical circular tetrad structure; (2) The chloroplast genome
of Borneo red bean contains 142 genes, including 97 protein coding genes ( PCG), 8 ribosomal RNA genes (rRNA), and 37 transport RNA genes (tRNA); (3) A total of 28225 codons were detected in the chloroplast
genome of Borneo red beans, with the highest number of codons encoding leucine (Leu), serine (Ser), and
arginine (Arg), all of which reached 6 types. The most frequently used codon was the AUU (1241) codon
encoding isoleucine ( Ile); (4) The nucleotide diversity analysis results indicate that the nucleotide diversity
levels of the four populations of Borneo red beans are relatively low. |
| Key words: chloroplast genome sequencing characterization Ormosia boluoensis |
