| 摘要: |
| 为完善任豆 Zenia insignis 组培快繁技术, 研究采用微枝扦插的组培再生方式, 探讨了基本培
养基中 NO
-
3 、 K
+
、 Ca
2+不同含量, 以及不同植物生长调节剂种类与浓度对任豆组培芽增殖与生根效果的
影响, 结果表明: (1) 任豆种子用 75%酒精、 0. 1%升汞分别浸泡灭菌 10, 30 min, 再用始温为 85 ℃无
菌水浸种 24 h 效果最好, 种子萌芽快, 种子发芽率可达 92. 16%; (2) RD 基本培养基中 NO
-
3 、 K
+
、 Ca
2+
含量分别以 600, 360, 330mg / L 为最优配比, 最佳继代培养基配比为 RD+6-BA 0. 2 mg / L+NAA 0. 01
mg / L, 20 d 增殖系数可达 3. 32; (3) NAA 对任豆组培芽生根以及生长有极显著的影响, 最佳生根培养
基配比为 1 / 2 MS+NAA 0. 5 mg / L+AC 1. 0 g / L, 生根率可达 100%, 且根系粗壮发达, 组培苗生长健壮,
叶片浓绿舒展。 |
| 关键词: 任豆 组培 微枝扦插 |
| DOI： |
| 分类号: |
| 基金项目:广东省林业科技创新项目（2023KJCX002） |
|
| Primary Study on Tissue Culture Regeneration Technology of Zenia insignis |
|
zhang xiaoming,Deng Xiaomei
|
|
1.Guangzhou Chongyao Enterprise management Consulting Co., LTD;2.College of Forestry and Landscape Architecture,South China Agricultural University
|
| Abstract: |
| In order to improve the tissue culture regeneration technology of Zenia insignis, the effects of
different contents of NO
-
3 , K
+
, and Ca
2+
in basic medium and different types and concentrations of plant growth
regulators on the proliferation and rooting of Z. insignis were investigated by means of micro-cuttings. The results showed as follows: (1) The seeds of Z. insignis were soaked with 75% alcohol for 10 min and 0. 1%
HgCl
2
for 30 min, respectively, and then soaked with sterile water at 85 ℃ for 24 h, which had the best effect,
rapid seed germination and seed germination rate can reach 92. 16%. (2) The optimal ratio of NO
-
3 , K
+
and
Ca
2+
in RD basic medium was 600 mg / L, 360 mg / L and 330mg / L, respectively. The optimal subculture medium was RD + 6-BA 0. 2 mg / L + NAA 0. 01 mg / L, and the proliferation coefficient reached 3. 32 at 20 days.
(3) NAA had a very significant effect on the rooting and growth of Z. insignis Chun. The optimal rooting medium was 1 / 2MS + 0. 5 mg / L NAA+ AC 1. 0 g / L, the rooting rate could reach 100%, and the roots were robust
and developed, the tissue culture seedlings grew robustly, and the leaves were dark green and stretched. |
| Key words: Zenia insignis tissue culture micro-cuttings |
