| 摘要: |
| 为了筛选高州油茶 Camellia gauchowensis 种子筛选最优贮藏方式, 研究采用 5 种方式进行贮藏
处理, 比较贮藏后的萌发特性和幼苗生长指标。 结果表明: (1) 不同贮藏处理的种子萌发特性存在显著
差异 (P<0. 05), 发芽率和发芽势均随贮藏时间推移而降低, 出苗率除贮藏 2 个月与 3 个月的种子间相差
不大以外, 其他也随贮藏时间延长呈现下降趋势。 -79 ℃超低温处理效果最佳, 其发芽率、 出苗率和发芽
势均高于其他处理, 种子的贮藏期可达 220 d。 常温、 蜡封液 A (吗啉脂肪酸盐果蜡)、 塑封、 蜡封液 B
(RQT-G-4 水果保鲜保湿增亮剂) 处理的种子在贮藏了 101 d 时, 胚芽生长缓慢, 保存了 162 d 后失活,
且蜡封液 A、 塑封、 蜡封液 B 处理的指标均显著高于常温处理。 4 ℃低温处理和使用蜡封液 C (橘子保鲜
剂) 蜡封处理的种子贮藏表现次之, 相较于常温等处理, 这两种处理延长了约一个月的保质期。 (2) 不
同贮藏处理的种子胚芽长、 胚根长、 胚芽直径和胚根直径均有显著差异, 且随着贮藏时间推移而降低。 -
79 ℃超低温处理的指标均显著高于其他贮藏处理, 其幼苗生长指标随时间的平均下降幅度最小, 贮藏效
果最佳; 蜡封液 C 处理种子的胚芽长、 胚根长、 胚芽直径和胚根直径显著高于常温处理; 常温处理效果
最差, 说明其幼苗生长能力受到最大影响, 而其他贮藏方式在一定程度上减少了外界环境对其幼苗生长发
育的影响。 贮藏方式对高州油茶种子萌发及幼苗生长有显著影响。 -79 ℃ 超低温处理效果最佳, 但成本
高, 蜡封液 C 蜡封处理效果次之, 相较于常温处理能延长约 1 个月的保质期, 成本低, 在生产中具有一
定应用价值。 |
| 关键词: 高州油茶 种子贮藏 种子萌发 种子生长指标 |
| DOI： |
| 分类号: |
| 基金项目:广东省重点领域研发计划项目 (2020B020215003)。 |
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| Effects of Different Storage Methods on Seed Germination and Seedling Growth of Camellia gauchowensis |
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YUANSHAN1, LIU Zhen2, MA Chunhua2, FU Zhiqiang2, SHEN Chunhui2, XI Ruchun3,4
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1.College of Forestry and Landscape Architecture, South China Agricultural University;2.College of Forestry and Landscape Architecture, South China Agricultural University/Guangdong Key;3.1.College of Forestry and Landscape Architecture, South China Agricultural University/Guangdong Key;4.2. Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, Guangdong
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| Abstract: |
| This study aimed to investigate the effects of various storage methods on the germination characteristics and seedling growth indexes of Camellia gauchowensis, thereby identifying the optimal methods that
would provide a scientific basis for its preservation and utilization. The research employed C. gauchowensis seeds
as experimental materials, subjecting them to five storage treatments and comparing their germination character-istics and seedling growth metrics. The results showed that (1) There were significant differences (P<0. 05) in
the germination characteristics among seeds stored under different conditions. The germination rate and germination potential exhibited a declining trend with increased storage duration, except for a marginal difference between 2 and 3 months of storage. The -79 ℃ ultra-low temperature treatment proved most effective, with higher
germination rate, germination percentage, and germination potential compared to other treatments, enabling
seed viability up to 220 days. Conversely, seeds treated at normal temperature, wax sealing solution A (Morpholine fatty acid salts fruit wax), plastic packaging, and wax sealing solution B (RQT-G-4 Fruit preservation
and moisturizing brightening agent) were stored for 101 days, the seedling grew slowly, and they were inactivated after 162 days of storage. The indexes of wax sealing solution A, plastic packaging, and wax sealing solution B treatment were significantly higher than those of normal temperature treatment. The 4℃ low temperature
and wax sealing solution C (Orange Freshness Retainer) treatments displayed second-best seed storage properties, extending the shelf life by approximately one month compared to room temperature storage. (2) There
were significant variations in shoot length, radicle length, shoot diameter, and radicle diameter among seeds
stored using different methods, all diminishing over time. The -79 ℃ ultra-low temperature treatment showed
significantly superior indexes compared to other treatments, with the smallest decrease in seedling growth metrics
over time, thus proving to be the best storage option. Seeds treated with wax sealing solution C had significantly
greater shoot and root lengths, as well as diameters, than those kept at room temperature. The normal temperature treatment demonstrated the poorest effect, indicating its seedlings′ growth ability was most severely affected. Other storage methods, however, mitigated the external environment′s impact on seedling growth and development to some extent. In summary, the storage methods significantly influenced the seed germination and seedling growth of C. gauchowensis. The effect of -79 ℃ ultra-low temperature treatment emerged as the most efficacious method, albeit with a high cost. Wax sealing solution C treatment presented the next best alternative, offering about a one-month extension in shelf life compared to room temperature storage while being economically
viable. This makes it a promising candidate for practical applications in production activities. |
| Key words: Camellia gauchowensis seed storage seed germination seedling growth index |
