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生长调节剂对马大杂种相思开放式组培增殖的影响
李泽瑞, 卢艳平, 陈玉军, 李玫, 黄烈健
中国林业科学研究院热带林业研究所
摘要:
为实现马大杂种相思 Acacia mangium × A. auriculiformis 树种的开放组培增殖, 建立马大杂种 相思开放式组培增殖培养体系, 文章以马大杂种相思组培苗为试验材料, 在无蔗糖的条件下, 采用正交设 计研究生长调节剂 6-BA、 IBA、 NAA 对马大杂种相思芽增殖的影响。 结果表明: 不同质量浓度的 6-BA、 IBA、 NAA 对马大杂种相思的增殖有显著影响; 马大杂种相思开放组培最佳增殖培养基为: MS + 2. 5 mg· L - 1 6-BA + 0. 1 mg·L - 1 IBA + 1. 0 mg·L - 1 IAA + 0. 2 g·L - 1百菌清, 增殖倍数为 2. 97。 通过对不同生 长调节剂的种类和浓度进行组合, 可以在无蔗糖的培养基中实现马大杂种相思有效增殖。
关键词:  马大杂种相思  开放式组织培养  生长调节剂  增殖
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基金项目:中央级公益性科研院所基本科研业务费专项基金(CAFYBB2017MB009)
Effects of Growth Regulators on Acacia mangium × A. auriculiformis Proliferation in Open Tissue Culture
LI Zerui, LU Yanping, CHEN Yujun, LI Mei, HUANG Liejian
Research Institute of Tropical Forestry, Chinese Academy of Forestry
Abstract:
In order to realize the open tissue culture and proliferation of Acacia mangium × A. auriculiformis, an open tissue culture and proliferation cultrue system of A. mangium × A. auriculiformis was established. In this paper, A. mangium × A. auriculiformis were used as test materials. An orthogonal design was used to study the effects of growth regulators 6-BA, IBA, and NAA on the proliferation under the condition of no sucrose, aiming to establish A. mangium × A. auriculiformis open tissue culture proliferation culture systems. The results showed that different concentrations of 6-BA, IBA and NAA had significant effects on the proliferation of A. mangium × A. auriculiformis; the effects of different growth regulators on the proliferation of A. mangium × A. auriculiformis were as follows: IAA > 6-BA > IBA. The best growth medium for open tissue culture is: MS + 2. 5 mg·L - 1 6-BA + 0. 1 mg·L - 1 IBA + 1. 0 mg·L - 1 IAA + 0. 2 g·L - 1 chlorothalonil, the multiple of proliferatione achieved 2. 97. The research results lay an important foundation for the further establishment of a perfect open tissue culture system of Acacia, and also provide an important reference for the related research of other tree species.
Key words:  Acacia mangium×A. auriculiformis  Open tissue culture  Growth regulator  Proliferation