| 摘要: |
| 以红锥 Castanopsis hystrix 成年优树嫁接苗萌发新枝为试验材料，通过扦插育苗方法，分析
NO 供体（SNP）、清除剂（cPTIO）及 ABT1 处理下插穗生根效果、内源激素、活性氧化物与抗氧化能力
的变化。结果表明：供试 75~1 000 mg/L SNP、cPTIO、ABT1 及对照（清水）16 个处理中，以 300 mg/L
SNP 和 500~1 000 mg/L ABT1 处理下的生根率高，达 69.5%~73.6%，但以 300 mg/L 浓度 SNP 处理下的根
系质量及成活效果最好，平均根条数 9.5、根长 20.3 mm、成活率 99.8%。与其他处理相比，除 500~1 000
mg/L 浓度 ABT1 处理外，在 300 mg/L 浓度 SNP 处理下扦插 10~30 d 后插穗中内源 IAA 水平明显偏高，
但 ZR、GA、ABA 水平无显著变化，在扦插 30 d 生根插穗中可见明显增强的 IAA 组织化学定位。同时，
扦插 10 d 时在 300 mg/L 浓度 SNP 处理下 O2 · -
和 H2O2 含量、SOD 活性显著高于其他任一浓度的 cPTIO
和 ABT1 处理。研究认为，相较生产中常用 ABT1，NO 能有效促进红锥生根能力的提升及根系质量的改
善，这与生根过程中 NO 诱导的 IAA 水平上升及根原基形成期增加的 O2 · -
、H2O2 含量和 SOD 活性有关。 |
| 关键词: 红锥 不定根 信号物质 IAA 活性氧化物 |
| DOI： |
| 分类号: |
| 基金项目: |
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| Effects of Exogenous Substances on Rooting and Related Physiological and Biochemical Characteristics During Rooting of Castanopsis hystrix |
|
Wen Hanping1, Chen Jiayu1, Zhong Yuting2, Zhong Yuanfang2, Guo Yanqing3
|
|
1.Zijin County Ecological public welfare Forest Management Center;2.Zijin Forestry Research Institute;3.Guangdong Eco-Engineering Polytechnic
|
| Abstract: |
| Taking the new branches of grafted seedlings of adult superior trees of Castanopsis hystrix as
experimental materials, the effects of NO donor (SNP), scavenger (cPTIO), and ABT1 on rooting, endogenous
hormones, active oxides, and antioxidant capacity of cuttings were analyzed. The results showed that among the
16 treatments of 75-1 000 mg/L SNP, cPTIO, ABT1, and control (clear water), the rooting rate of 300 mg/L SNP
and 500-1 000 mg/L ABT1 was 69.5%-73.6%, but the root quality and survival effect of 300 mg/L SNP treatment
was the best, with average 9.5 roots, 20.3 mm root length and 99.8% survival rate. Compare with other treatments,
except 500-1 000 mg/L ABT1 treatment, the level of endogenous IAA in cuttings treated with 300 mg/L SNP for 10~30 days was significantly higher, but the consent rations of ZR, GA, and ABA did not change significantly.
The content of O2·
- and H2O2 and SOD activity under 300 mg/L SNP treatment were significantly higher than those
under other concentrations of cPTIO and ABT1 treatment. Compared with ABT1 commonly used in production,
NO could effectively promote the rooting ability and root quality of C. hystrix, which was related to the increased
IAA level induced by NO during rooting and the increased O2·
-
, H2O2 content and SOD activity during root
primordium formation. |
| Key words: Castanopsis hystrix adventitious root signal substance IAA reactive oxygen species |
