| 摘要: |
| 外来入侵物种曲纹紫灰蝶 Chilades pandava 是危害苏铁属的重要害虫。从植物中寻找驱避活性成分为曲纹紫灰蝶的绿色防控提供基础支撑。试验采用水蒸气蒸馏法提取番石榴 Psidium guajava 叶精油，对其化学组成进行气相色谱 - 质谱联用（GC-MS）分析，采用 Y 型嗅觉仪和气相色谱 - 触角电位联用仪（GC-EAD）测定精油及其主要成分对曲纹紫灰蝶雌成虫的驱避作用和触角电位活性。研究结果表明，番石榴叶精油含有大量的倍半萜及其氧化物，主要是 (E)-β- 石竹烯（32.47%）、α- 古巴烯（10.44%）、菖莆烯（8.19%）、蓝桉醇（7.19%）和香橙烯（5.01%）等。曲纹紫灰蝶对番石榴叶精油中的 8 种成分有触角电位反应，其中对 (E)-β- 石竹烯的反应最明显。番石榴叶精油和 (E)-β- 石竹烯均能驱避曲纹紫灰蝶，驱避率与浓度呈正相关，在 0.5 μL/mL 时，驱避率分别为 73.08% 和 68.00%。 |
| 关键词: 曲纹紫灰蝶 植物精油 化学成分 驱避活性 触角电位 |
| DOI： |
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| 基金项目:广东省林业科技创新项目（2019KJCX025）；广东省基础与应用基础研究基金（2019A1515110596） |
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| Repellent Activity and Antennal Electrophysiological Responses of Essential Oil from Psidium guajava Leaves Against Chilades pandava |
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HUANG Hua1, LING Siquan2, QIU Hualong2, ZHONG Jiamei2, XU Jinzhu2
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1.Forest Pest Control and Quarantine station,Fengkai County Forestry Bureau,Zhaoqing;2.Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou
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| Abstract: |
| The invasive alien species Chilades pandava is a major insect pest of Cycas spp. plants. Repellents from non-host plants provide a basis for the green control of C. pandava. In this study, the essential oil of Psidium guajava leaves (PGEOs) was extracted with the steam distillation method, and its chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). The repellent effect and the electroantennographic activity of PGEOs as well as its main components on the females of C. pandava were determined with the Y-olfactometer and the gas chromatography-electroantennographic detection (GC-EAD) respectively. The results from the GC-MS analysis showed that PGEOs mainly contained sesquiterpenes and oxygenated sesquiterpenes, including (E)-β-caryophyllene (32.47%), α-copaene (10.44%), calamenene (8.19%), globulol(7.19%) and aromandendrene(5.01%). GC-EAD recordings confifirmed the electrophysiological activity of eight volatile compounds in PGEOs, of which(E)-β-caryophyllene was the most active compound and had a dose-dependent effect. PGEOs and (E)-β-caryophyllene can both signifificantly repel C. pandava females with dose-dependent, and the repellent rates were 73.08% and 68.00%, respectively, at the concentration of 0.5 μL/mL. |
| Key words: Chilades pandava essential oils chemical constituents repellent activity electroantennogram |
