| 摘要: |
| 研究以木荷 Schima superba 无性系清远 101 花枝及花朵为试验材料，采用 TTC 染色鉴定法，
研究不同培养液培养木荷离体花枝的花粉活力和不同贮藏温度、湿度和堆积高度的花粉活力变化规律。
研究表明：5 d 短期内，MS 或 10% 蔗糖有利于木荷花枝培养，花枝中花粉活力较高；4℃以下低温，70%
空气湿度和 1.5 cm 低堆积高度更有利于花药中的花粉保存。结论：花枝在 MS 和 10% 蔗糖培养液中持续
培养，短时间内具有较高的花粉活力，能满足木荷授粉需要；常温下花粉保存在 70% 中等湿度或较低堆
积高度能维持较高的活性；木荷花粉的长期保存需要低温或超低温进行保存。 |
| 关键词: 木荷 花粉活力 贮藏条件 |
| DOI： |
| 投稿时间：2022-08-19修订日期：2022-12-05 |
| 基金项目: |
|
| Effects of Different Storage Conditions on Pollens of Schima superba |
| ZHU Gang,YAO Jun,HE Bo-xiang,LIANG Dong-cheng,CAI Yan-ling,ZHANG Qian |
| (Zhongkai University of Agriculture and Engineering,Guang Zhou,Guang Dong) |
| Abstract: |
| The pollen viability of isolated ?owering branches and ?owers of Schima superba asexual line
Qingyuan 101 was used as test materials, and the TTC staining identi?cation method was used to study the pollen
viability of isolated ?owering branches of S.superba cultured in different culture solutions and the change patterns
of pollen viability at different storage temperatures, humidity and stacking heights. The results showed that: In the
short term of 5 days, MS or 10% sucrose was bene?cial to the ?ower branch culture of S.superba, and the pollen
activity in the ?ower branch was higher; Low temperature below 4 ℃ , 70% humidity and 1.5 cm low stacking
height are more conducive to the preservation of pollen in anthers. Conclusion: ?owering branches of MS and
10% sucrose culture solution continuously cultivated with high pollen viability for a short period of time can
meet the pollination needs of S.superba; pollen preservation at 70% medium humidity or low stacking height at
room temperature can maintain high activity; long-term preservation of S.superba pollen requires low or ultra-low
temperature for preservation. |
| Key words: Schima superba pollen vitality storage conditions |
