| 摘要: |
| 2020 年 8 月， 广 东 省 野 生 动 物 监 测 救 护 中 心 救 护 的 一 只 成 体 雌 性 中 华 穿 山 甲 Manis
pentadactyla，在人工饲养条件下自然受孕，妊娠约 11 周后产出无生命体征的双胞胎。在合圈前及孕期，
收集雌性的粪便，采用酶联免疫法（Elisa），测定激素促黄体素、促卵泡素、催乳素、孕激素、睾酮和雌
二醇的含量。结果发现，雌二醇水平在妊娠第 7 周达到最高（1 050.14 pmol/L）；促黄体素水平在妊娠第
4 周达到最高（53.95 mIU/mL）；促卵泡素水平在妊娠第 5 周达到最高（26.08 mIU/mL）；孕激素含量在
妊娠第 2 周达到最高（22.57 ng/mL）后波动性下降；睾酮水平呈波动变化，但在合圈前后无明显差异；
催乳素水平在流产当天最低（797.15 mIU/L）。两胚胎体重分别为 25.4 g 和 18.3 g，总长分别为 15.8 cm 和
13.6 cm；头部、躯干、尾部和四肢均分化良好，但有差异，鳞片覆盖背部、尾部和部分四肢；四爪形状
良好，未角质化；眼部未发育完全；唇和舌头具备形态；肋骨具备明显轮廓；可看到部分肠道形状。利
用微卫星标记技术鉴定其为异卵双胞胎。 |
| 关键词: 人工饲养 中华穿山甲 双胞胎 异卵 |
| DOI： |
| 分类号: |
| 基金项目:广东省林业科技创新项目 |
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| A Case of Surveillance of Hormones during Pregnancy and Identification of Twins in Captive Chinese Pangolin |
|
yanhongmei1, xuxuelin2, anfuyu2, wangkai2, xiayanling1, huayan2
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1.Northeast Forestry University;2.guangdong academ of forestry
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| Abstract: |
| In August 2020, an adult female Chinese pangolin (Manis pentadactyla) was pregnant, and gave birth to inanimate twins after 11 weeks of gestation, which was rescued by Guangdong Wildlife Monitoring and Rescue Center. The levels of estradiol, testosterone, luteinizing hormone, follicle stimulating hormone, progesterone and prolactin in the feces of female pangolin were determined by enzyme linked immunosorbent assay. The results showed that estradiol levels peaked at 1050.14 pmol/L after mating seven weeks; luteinizing hormone levels peaked at 53.95 mIU/mL after mating four weeks; follicle stimulating hormone levels peaked at 26.08 mIU/mL after mating five weeks; the progesterone content reached the peak at 22.57ng/mL after mating two weeks and the volatility decreased; testosterone levels have been fluctuated, no significant different at before and after mating; prolactin reached its lowest point on the day of abortion (797.15 mIU/L). The stillbirth had well differentiated, and the morphological measurements showed that their weights were 25.4 g and 18.3 g, and their total lengths were 15.8 cm and 13.6 cm, respectively. Head, trunk, tail and limbs all morphological, with scales covering excepted head and parts of limbs. The claws bent and soft. Their eyes hypoplasia, lips and tongue well developed. The ribs and bowel shape clearly seen. They were identified as fraternal twins using microsatellite marker technology. This case provides reference materials for both the developmental biology and artificial breeding of Chinese pangolins. |
| Key words: captivity Manis pentadactyla twins dizygotie |
