| 摘要: |
| 为研究干旱胁迫条件下屋顶绿化植物小叶黄杨叶片酶活性的变化特征，本文通过盆栽试验模拟自然干旱条件，设置正常供水（CK）、轻度干旱胁迫（TB）、中度干旱胁迫（TC）和重度干旱胁迫（TD）4个处理，动态监测不同生长时期小叶黄杨叶片相关酶活性。结果表明，随着干旱胁迫程度的增加和时间的延长，叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）活性呈现升高-降低-升高趋势；小叶黄杨叶片丙二醛（MDA）含量呈先下降后上升趋势；过氧化氢酶（CAT）活性和可溶性蛋白含量变化呈先上升后下降趋势。可见，轻度干旱胁迫会使小叶黄杨抗氧化酶活性增强和可溶性蛋白含量升高，但严重的干旱胁迫会损害其正常生长，研究结果为小叶黄杨更好适应屋顶种植环境提供理论依据，以期为我国屋顶绿化的研究和推广提供指导。 |
| 关键词: 小叶黄杨 屋顶绿化 干旱胁迫 叶片酶活性 抗氧化 |
| DOI： |
| 分类号: |
| 基金项目:河南省产学研项目“基于立体绿化轻质生态基质的研发”（142107000084） |
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| Effects of Drought Stress on Antioxidant Properties of Roof Greening Plant Buxus microphylla Leaves |
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LIU Hui, ZHANG Chongyang, LIU Shiliang, ZHOU Mengliang, WU Miaomiao, JIANG Guiying, LIU Fang, ZHAO Ying
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College of Resources and Environment, Henan Agricultural University
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| Abstract: |
| The pot experiment was carried out with taking the leaves of 2-year-old B. microphylla as test materials under simulated natural drought conditions. Four treatments of normal water supply (80%-90%) field water capacity，CK), mild drought stress (60%-70% field water capacity，TB), moderate drought stress (50% -60% field water capacity，TC) and severe drought stress (35% -45% field water capacity，TD) were set to dynamically monitor the activities of related enzymes in leaves of B. microphylla at different growth stages, in order to study the changes characteristics of leaf antioxidant properties of B. microphylla which was a roof greening plant under drought stress.The study found that the activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves were increased first, then decreased, and finally increased with drought stress degree and time.The content of malondialdehyde (MDA) in leaves of B. microphylla was decreased firstly and hen increased. The activity of catalase (CAT) and the content of soluble protein were increased firstly and then decreased. So the activity of antioxidant enzymes and the content of soluble protein increased under mild drought stress, but the normal growth of B. microphylla would be damaged under severe drought stress.The results showed that the relative balance of oxide content was coordinated and maintained by increasing protective enzyme activity and soluble protein content to ensure the normal growth and development of B. microphylla after mild
and moderate drought stress, while after severe stress, the protective enzyme system and cells of B. microphylla suffered irreversible damage and affected their normal growth. |
| Key words: Buxus microphylla roof greening drought stress leaf enzyme activity characteristics antioxidant |
