| 摘要: |
| 拮抗菌株 SY-15 是从杨树根际土壤中分离获得的一株能高效拮抗金黄壳囊孢菌 Cytospora. chrysosperma 的拮抗细菌。研究基于形态学特征、生理生化特性和 16S rDNA 序列分析鉴定了菌株 SY-15的分类地位，并分别采用带药平板法和二分皿法分析了其抑菌活性，为进一步开发利用拮抗菌株 SY-15 提供科学依据。结果表明：拮抗菌株 SY-15 最终鉴定为枯草芽孢杆菌 Bacillus subtilis，其无菌培养滤液和挥发性气体均能有效抑制金黄壳囊孢菌、迂回壳囊孢菌 C. ambiens 和黑腐皮壳菌 C. mali 的生长，处理 60 h后，无菌培养滤液对 3 种植物病原真菌的抑菌率分别达到 88.89%、60.39% 和 53.02%。处理 36 h 后，挥发性气体对 3 种植物病原真菌的抑菌率分别达到 100%、76.98% 和 63.41%。此外，还发现拮抗菌株 SY-15 能产生蛋白酶和纤维素酶。研究表明拮抗菌株 SY-15 是一株潜在的高效防治杨树腐烂病及其他植物真菌病害的菌株资源。 |
| 关键词: 植物病原真菌 枯草芽孢杆菌 鉴定 抑菌活性 挥发性气体 |
| DOI： |
| 分类号: |
| 基金项目:广东省林业科技创新项目 (2020KJCX004)，广东省基础与应用基础研究基金 (2019A1515011814) |
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| Identification of Antagonistic Strain SY-15 and Analysis on Its Antifungal Activity |
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huanghuayi, HU Lili, CHEN Liusheng, Zhao Danyang, HUANG Yonghuai
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Guangdong Academy of Forestry
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| Abstract: |
| The antagonistic strain SY-15 is an antagonistic bacteria isolated from the rhizosphere soil of poplar plant that effectively antagonize Cytospora chrysosperma. In order to provide technical guidance for further exploitation and utilization of strain SY-15, the species of strain SY-15 was identified based on morphological and physiological characteristics, as well as a phylogenetic analysis of partial 16S rDNA sequence. The antifungal activity of SY-15 were analyzed by colony diameter assay and two-compartment plate method. The results showed that strain SY-15 was identified as Bacillus subtilis. Sterile culture filtrates and volatiles produced by strain SY-15 showed strong antifungal activity against three fungal phytopathogens (C. hrysosperma, C. ambiens and C. mali). After 60 hours of treatment, the inhibition rates of sterile culture filtrates against C. chrysosperma, C. ambiens and C. mali were 88.89%, 60.39% and 53.02%, respectively. After 36 hours of treatment, the inhibition rates of volatiles against C. chrysosperma, C. ambiens and C. mali were 100%, 76.98% and 63.41%, respectively. In addition, it was also found that strain SY-15 had activities of protease and cellulase. This study indicate that the antagonistic strain SY-15 is a potential strain resource for effective control of poplar canker and other plant fungal diseases. |
| Key words: fungal phytopathogens Bacillus subtilis identification antifungal activity volatiles |
