| 摘要: |
| 火炬松Pinus taeda 是一种生长迅速、适应性强的针叶用材树种，针对火炬松针叶和树皮组织中的代谢产物的利用开发仍处于初级阶段，因此探究火炬松针叶和树皮组织的代谢产物具有重要的实际意义。研究利用超高效液相色谱和质谱联用技术（UPLC-MS/MS）对火炬松的针叶和树皮中的代谢物成分进行鉴定，并采用主成分分析、正交偏最小二乘法分析、聚类和富集分析等方法对火炬松针叶和树皮中的代谢物成分进行比较分析。结果表明，从火炬松的针叶和树皮中共鉴定出523种代谢物成分，主要为氨基酸类、酚酸类、有机酸类、鞣质、脂质类和黄酮类等化合物。火炬松针叶和树皮组织部位之间代谢物成分含量差异显著，其中槲皮素、矢车菊素、原花青素、丁香树脂酚、表儿茶素等物质在树皮组织中的含量显著高于针叶组织，而柚皮素、山姜素、山奈酚、泽兰黄素、秦皮甲素等物质则在火炬松针叶组织中含量更高。此外，302 种含量差异显著的代谢物显著富集于类黄酮生物合成通路、黄酮与黄酮醇生物合成通路、氨酰tRNA生物合成通路、氨基酸生物合成通路及淀粉和蔗糖代谢通路。 |
| 关键词: 火炬松 UPLC-MS/MS 代谢组分析 |
| DOI： |
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| 基金项目:广东省重点领域研发计划资助（2020B020215001） |
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| Comparative Analysis of Metabonomics Between Needles and Bark of Pinus taeda |
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zeng wei shan1, huang lin wang1, chen man yu1, mao ji peng2, huang shao wei1, ning hua gong3, liu tian yi1
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1.South China Agricultural University;2.Taishan Hongling Seed Orchart;3.Yingde Institute of Foresty
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| Abstract: |
| Pinus taeda is a fast-growing and highly adaptable coniferous tree species. The exploitation of
metabolites in the needles and bark tissues of P. taeda is still in its early stages, so it is of practical importance to investigate the metabolites in the needles and bark tissues of P. taeda. In this study, UPLC-MS/MS was used to identify the metabolites in the pine needles and bark of P. taeda, and the principal component analysis, orthogonal partial least squares analysis, and polymerization were used to identify the components of the metabolites in the pine needles and bark of P. taeda. Methods such as class and enrichment analysis are used to compare and analyze the composition of their metabolites. The results showed that a total of 523 metabolite components were identifified from the bark and pine needles of P. taeda, mainly including amino acids, phenolic acids, organic acids, tannins, lipids and flflavonoids. There are signifificant differences in the content of metabolites between bark and pine needle tissue of P. taeda. Among them, the content of quercetin, cyanidin, proanthocyanidin, eugenol, and epicatechin in the bark tissue were significantly higher than that in the pine needle tissue, while naringenin, kaempferin, kaempferol, zearanthin and aesculin were higher in needle tissue. In addition, 302 metabolites with signifificantly different contents were signifificantly enriched in the flflavonoid biosynthesis pathway, biosynthesis of secondary metabolites pathway, biosynthesis of pathway amino acids, and starch and sucrose metabolism pathway. |
| Key words: Pinus taeda UPLC-MS/MS metabonomic analysis |
