| 摘要: |
| 为了解大亚湾核心区游泳动物卵和幼体的种类和数量，研究于2020年 5月和10月对大亚湾3个重要的游泳动物卵和幼体分布较为密集区域（27个站位）开展了定量取样调查。5月共采集卵和幼体1 660尾（粒），经COI鉴定分析，共鉴定出84种，隶属于4门11目48科64属，其中包含鱼类60种，虾类9种，蟹类12种，软体动物1种；10月共采集卵和幼体1 580尾（粒），经COI鉴定分析，共鉴定出62种，隶属于2门11目40科53属。其中包含鱼类47种，虾类10种，蟹类4种，虾蛄1种。对比历史数据，鉴定结果表明DNA 条形码技术是传统形态学强有力的补充，是一种准确的、规范化的、有效的物种鉴定工具。 |
| 关键词: DNA条形码 大亚湾 游泳动物 卵 幼体 分类鉴定 |
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| Application of DNA Barcoding in Classification and Identification of Nekton Eggs and Larvae from Daya Bay Coastal |
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zhangtaoli1, liqingyong2, zhangzhimin1, yeguan3, qianhongbiao1, linhaiqiang2
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1.Guangdong Daya Bay Aquatic Resources Provincial Nature Reserve Management Office;2.Huizhou Fishery Research and Extension Center;3.Huizhou Zhihong Marine Technology Co., Ltd.
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| Abstract: |
| In order to understand the species and quantity of nekton eggs and larvae in the core area of Daya Bay, a quantitative sampling survey was conducted in May and October 2020 in three areas (27 stations) where the distribution of nekton eggs and larvae was relatively dense. A total of 1 660 nekton eggs and larvae were collected in May. A total of 84 species were identified by COI analysis, belonging to 4 phyla, 11 orders, 48 families and 64 genera, including 60 species of fish, 9 species of shrimp, 12 species of crab and 1 species of mollusk. A total of 1580 eggs and larvae were collected in October. A total of 62 species were identified by COI analysis, belonging to 2 phyla, 11 orders, 40 families and 53 genera. There were 47 species of fish, 10 species of shrimps, 4 species of crabs and 1 species of mantis shrimps. Compare to historical data, our results demonstrate that being a powerful complement to traditional morphology, DNA barcoding is an accurate, standardized, and efficient tool for species identification, and possesses a broad prospect of application in fish biodiversity research |
| Key words: DNA barcoding Dayawan Bay nekton egg larva classification and identification |
