| 摘要: |
| 以嘉氏羊蹄甲 Bauhinia galpinii 带腋芽茎段为外植体，建立组织培养快繁体系。结果表明：
最适合的嘉氏羊蹄甲带腋芽茎段诱导的培养基为：MS+0.5 mg · L-1 6-BA+0.05 mg · L-1 NAA，诱导率为
80.00%；最佳增殖培养基为 :WPM+0.50 mg · L-1 6BA+0.40 mg · L-1IAA，增殖系数达 4.62；最佳生根培养
基为：1/2MS+1.00 mg · L-1 IBA +0.50 mg · L-1NAA+0.10 mg · L-1 IAA +15 g · L-1 蔗糖 +7 g · L-1 卡拉胶，生
根率达 98.00%；最佳移栽基质为：V（黄心土）：V（泥炭）：V（珍珠岩）＝ 1:3:1，试管苗移栽成活率达
86.7%。试验建立了嘉氏羊蹄甲组织培养体系，提高了嘉氏羊蹄甲增殖率、生根率和移栽成活率。 |
| 关键词: 嘉氏羊蹄甲 组织培养 正交试验 增殖培养 |
| DOI： |
| 分类号: |
| 基金项目:广东省林业科技创新项目“宫粉紫荆优良品系选育及高效栽培技术研究与示范”（2018KJCX043）；广州市科技计划项目“宫粉紫荆种质资源遗传分析及优良品种筛选”（201300000105）；广州市林业和园林局财政预算科研项目“羊蹄甲属观花植物资源引种收集与良种选育研究”（穗林业和园林局[2015]73号 |
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| Establishment of Tissue Culture System of Bauhinia galpinii |
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HE Chaoyin,WEI Dan,TANG Honghui,XU Chongyong,LAN Yanqun,fengzhijian,chenyouguang,zhanggeng
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1.Guangdong Academy of Forestry, Guangdong Provincial Key Laboratory of Silviculture Protection and Utilizationy;2.College of Forestry and Landscape Architecture, South China Agricultural University,;3.Guangzhou Institute of Forestry and Landscape Architecture
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| Abstract: |
| The tissue culture system of Bauhinia galpinii with axillary buds was established. The results
showed that the impact of.the most suitable medium for the induction of stem segment with axillary bud was MS +
0.5 mg · L-1 6-BA + 0.05 mg · L-1NAA, the induction rate was 80.00%; the best proliferation medium was WPM +
0.50 mg · L-1 6BA + 0.40 mg · L-1 IAA, the proliferation coefficient was 4.62; the best rooting medium was 1 /
2 ms + 1.00 mg · L-1 IBA + 0.50 mg · L-1 NAA + 0.10 mg · L-1 IAA + 15 g · L-1 sucrose + 7 g · L-1 carrageenan,
the rooting rate was 98.00%; the best transplanting medium was V (yellow soil): V (peat): V (perlite) = 1:3:1, the
survival rate of plantlets transplanting was 86.7%. The tissue culture system was established, which could improve
the proliferation rate, rooting rate and transplanting survival rate. |
| Key words: Bauhinia galpinii tissue culture orthogonal test proliferation culture |
