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圈养华南虎血液生化指标正常参考值初步研究*
任小冬1, 黄勉2, 李石洲1, 雷胜桥1, 虞坚颐3, 刘力军1
1.广东粤北华南虎省级自然保护区管理处;2.广州动物园;3.上海动物园
摘要:
以国内部分圈养的健康华南虎Panthera tigris amoyensis 作为研究对象,通过收集华南虎血液生化报告和采集华南虎血液样本检测建立样本数据,血样检测采用自动血液生化分析仪干式试剂片生化技术测定,测定血液生化指标包括血糖(GLU)、总蛋白(TP)、总胆固醇CHOL)、总胆红素(TBIL)、肌酐(CREA)等。得到华南虎血液生化共38 项指标的正常参考值范围(95% 概率),其中几项主要指标正常值范围如下:GLU(2.82-11.55)mmol/L、TP(56.58-85.93)g/L、CHOL(3.32-8.66)μmol/L、TBIL(0-5.30)μmol/L、CREA(52-349)mol/L 等。统计结果显示,雌雄华南虎间血液生化指标的部分项目有差异(P <0.05),幼龄组和成年组间各项生化指标差异不显著(P >0.05),与其他虎亚种相关血液生化报道的生化指标部分有差别。华南虎血液生化指标正常参考值范围不同于其他虎亚种,建立华南虎血液生 化指标正常值对华南虎的饲养管理、疾病诊治预防和保育繁殖研究工作有重要作用。
关键词:  华南虎  血液生化  正常参考值
DOI:
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基金项目:韶关市科技计划项目(2014CX/K347,2017CX/K050)
A Preliminary Study on Normal Reference Values of Blood Biochemical Indexes of Captive Panthera tigris amoyensis
REN Xiaodong1, HUANG Mian2, LI Shizhou1, LEI Shengqiao1, YU Jianyi3, LIU Lijun1
1.Procincial Nature Management Service of South China Tigers in Northern Guangdong;2.Guangzhou Zoo,Guangzhou;3.Shanghai Zoo,Shanghai
Abstract:
In this study, the normal reference values of blood biochemical indexes of domestic captive Panthera tigris amoyensis were studied. Taking some domestic captive healthy Panthera tigris amoyensis as the research object, Sample data were established by collecting blood biochemical reports and collecting blood samples of Panthera tigris amoyensis. Blood samples were detected by automatic blood biochemical analyzer dry reagent tablet biochemical technology. Blood biochemical indexes including blood sugar (GLU), total protein (TP), total cholesterol (CHOL), total bilirubin (TBIL), creatinine (CREA) were determined. The normal reference ranges (95% probability) of 38 blood biochemical indices of Panthera tigris amoyensis were obtained. including: GLU (2.82-11.55) mmol/L, TP (56.58-85.93) g/L, CHOL (3.32-8.66) μmol/L, TBIL (0-5.30) μmol/L, CREA (52-349) μmol/L, etc. The statistical results showed that there were differences in some items of blood biochemical indexes between male and female Panthera tigris amoyensis (P < 0.05), and there was no significantdifference in biochemical indexes between the young group and the adult group (P > 0.05),which were different from the biochemical indexes reported by other subspecies of tigers. The reference range of blood biochemical parameters of Panthera tigris amoyensis is different from that of other tiger subspecies. Establishing normal blood biochemical parameters of Panthera tigris amoyensis plays an important role in feeding management, disease diagnosis, prevention, conservation and reproduction of Panthera tigris amoyensis.
Key words:  Panthera tigris amoyensis  blood biochemical indexes  reference range