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油茶象甲对油茶果实挥发物的触角电生理和行为反应
曾家城1, 秦长生2, 徐金柱2, 赵丹阳2, 杨华2, 田龙艳2, 邱华龙2
1.广东省肇庆市广宁县林业局;2.广东省林业科学研究院 广东省森林培育与保护利用重点实验室
摘要:
寄主植物挥发性化合物往往对昆虫搜寻定位寄主及食物选择具有重要的影响，为了明确油茶 （Camellia oleifera）果实中是否含有引诱油茶象甲（Curculio chinensis）取食的特定挥发性化合物，研究 利用 GC-EAD 测试了油茶果实挥发物对油茶象甲的触角电位反应，通过 GC-MS 数据库对活性挥发物进 行鉴定，并采用 Y 型嗅觉仪测试了油茶象甲对活性化合物的行为反应。研究结果表明在 15.05、19.30、 21.97 和 22.79 min 4 个出峰时间点的物质能够引起油茶象甲较强的触角反应，经过质谱鉴定和标准品比 对，确定在 15.05 和 19.30 min 出峰的物质分别为正十八烷和油酸酰胺。Y 型嗅觉仪选择试验结果表明 0.1 和 1 ng/μL 浓度的正十八烷对油茶象甲具有较强的驱避作用，1 ng/μL 的油酸酰胺对油茶象甲具有较强的 引诱作用。研究确定了正十八烷和油酸酰胺对油茶象甲的行为具有调控作用。
关键词:  油茶象甲  GC-EAD  行为反应  植物挥发物  信息素
DOI：
分类号:
基金项目:广东省自然科学基金(2018A030310690)和广东省地方标准制定项目(2018-DB-01)。。
Electroantennogram and Behavioral Responses of Curculio chinensis to Volatiles of Camellia oleifera Fruits
ZENG Jiacheng1, QIN Changsheng2, XU Jinzhu2, ZHAO Danyang2, YANG Hua2, TIAN Longyan2, QIU Hualong2
1.Forestry Administration of Guangning County,Zhaoqing,Guangdong;2.Guangdong Academy of Forestry Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou
Abstract:
The volatile compounds of host plants could influence the host plants location and food searching of insects. In order to find out whether there is any volatile compound of Camellia oleifera fruits that attract Curculio chinensis, in this study, GC-EAD was used to test the electroantennogram response of C. chinensis to the volatile compounds of C. oleifera fruits, GC-MS was used to identify the active volatile compounds, and Y-olfactometer was used to test the behavioral responses of C. chinensis to purchased pure compound. The results showed that at the retention time of 15.05, 19.30, 21.97 and 22.79 min, these four compounds could cause strong antennae reaction of C. chinensis. After identifying by mass spectrometry comparing with standard compounds, the peaks at retention time of 15.05 and 19.30 min were determined as n-octadecane and oleamide, repectively. The results of Y-olfactometer showed that 0.1 ng/μL and 1 ng / μL of n-octadecane had strong repellent effect against C. chinensis, and 1 ng/μL of oleamide showed significantly attractive effect on C. chinensis. In this study, we showed that n-octadecane and oleamide had a regulatory effect on the behavior of C. chinensis.
Key words:  Curculio chinensi  GC-EAD  behavioral regulation  plant volatiles  pheromone