香花崖豆藤组织培养技术

陈颖乐; 曾雷; 王彩云; 王颂; 徐巧林; 余玉娟; 黄文妍

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香花崖豆藤组织培养技术
陈颖乐¹, 曾雷¹, 王彩云², 王颂², 徐巧林², 余玉娟², 黄文妍²
1.广东省森林培育与保护利用重点实验室/广东省林业科学研究院;2.1

摘要:

以香花崖豆藤（Millettia dielsiana）茎段为外植体，通过双因素试验和正交试验分别对茎段腋芽增殖、生根的影响因素进行筛选，建立了香花崖豆藤的快速繁殖体系。结果表明：香花崖豆藤茎段腋芽增殖培养最佳培养基为 NAA 0.1 mg · L-1 + 6-BA 0.8 mg · L-1 MS；最适生根培养基为 IBA 0.5 mg · L-1、 NAA 0.6 mg · L-1、GGR6 mg · L-1，生根率为 86.0%，形成了可以进行产业化的香花崖豆藤组织培养技术。

关键词: 香花崖豆藤腋芽组织培养生根率

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Tissue Culture Technology of Millettia dielsiana

chen ying le¹, zeng lei¹, 1², 1², 1², 1², 1²

1.Guangdong Academy of Forestry;2.1

Abstract:

Using stem segment of Millettia dielsiana as explants, the influence factors of axillary bud proliferation and rooting in stem segment were screened by two-factor test and orthogonal test, and the rapid propagation system of stem segment was established. The results showed that NAA 0.1 mg · L-1 + 6-BA 0.8 mg · L- 1 MS was the optimal medium for axillary bud proliferation culture of stem segment. The optimal rooting medium was IBA 0.5 mg · L1 , NAA 0.6 mg · L-1 and GGR6 mg · L-1, and the rooting rate was 86.0%. The technology of tissue culture of M. dielsiana has been developed.

Key words: Millettia dielsiana axillary bud tissue culture rooting rate

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