| 摘要: |
| 以香花崖豆藤（Millettia dielsiana）茎段为外植体，通过双因素试验和正交试验分别对茎段腋
芽增殖、生根的影响因素进行筛选，建立了香花崖豆藤的快速繁殖体系。结果表明：香花崖豆藤茎段腋
芽增殖培养最佳培养基为 NAA 0.1 mg · L-1 + 6-BA 0.8 mg · L-1 MS；最适生根培养基为 IBA 0.5 mg · L-1、
NAA 0.6 mg · L-1、GGR6 mg · L-1，生根率为 86.0%，形成了可以进行产业化的香花崖豆藤组织培养技术。 |
| 关键词: 香花崖豆藤 腋芽 组织培养 生根率 |
| DOI： |
| 投稿时间：2020-06-10修订日期：2020-06-29 |
| 基金项目: |
|
| Tissue Culture Technology of Millettia dielsiana |
| chen ying le,zeng lei,1,1,1,1,1 |
| (Guangdong Academy of Forestry;1) |
| Abstract: |
| Using stem segment of Millettia dielsiana as explants, the influence factors of axillary bud
proliferation and rooting in stem segment were screened by two-factor test and orthogonal test, and the rapid
propagation system of stem segment was established. The results showed that NAA 0.1 mg · L-1 + 6-BA 0.8 mg · L- 1
MS was the optimal medium for axillary bud proliferation culture of stem segment. The optimal rooting medium
was IBA 0.5 mg · L1
, NAA 0.6 mg · L-1 and GGR6 mg · L-1, and the rooting rate was 86.0%. The technology of
tissue culture of M. dielsiana has been developed. |
| Key words: Millettia dielsiana axillary bud tissue culture rooting rate |
