| 摘要: |
| 选用已经通过无性系测定的优良无性系单株尾巨桉（Eucalyptus urophylla × E. grandis） M8，
以其半木质化嫩枝为外植体，采用组培繁殖中遗传稳定性高的丛芽发生途径，探讨基本培养基、6-BA、
IBA、蔗糖和 pH 这 5 个因子分别对增殖效果的影响。结果表明：（1）采用 MS + 6-BA 0.2 mg/L + 蔗糖 30
g/L + 卡拉胶 6.8 g/L，pH 为 5.8 的培养基进行尾巨桉 M8 外植体腋芽的诱导时，10 ~ 15 d 为出芽高峰期，
平均污染率为 15.3%，平均诱导率为 77.9%；（2）适宜尾巨桉 M8 增殖的最佳培养基为改良 MS 培养基、
6-BA 浓度为 0.2 ~ 0.5 mg/L、IBA 浓度为 0.05 ~ 0.2 mg/L、蔗糖浓度为 30 g/L、pH 值范围为 5.4 ~ 6.0，继
代周期为 20 d，增殖系数可达 4.49，有效芽数可达 30 棵 / 瓶。因此，尾巨桉 M8 较适宜的诱导培养基
为：MS + 6-BA 0.2 mg/L + 蔗糖 30 g/L + 卡拉胶 6.8 g/L，可实现腋芽萌发快，污染率少，诱导率高。最优
的增殖培养基为改良 MS + 6-BA 0.2 mg/L + IBA 0.1 mg/L + 蔗糖 30 g/L + 卡拉胶 6.8 g/L，pH 为 5.4 ~ 6.0，
可实现继代苗叶片舒展，生长健壮，提高组培效率，发挥组培快繁的优势。 |
| 关键词: 桉树，组织培养，增殖，培养基，6-BA，IBA |
| DOI： |
| 投稿时间：2020-06-09修订日期：2020-06-09 |
| 基金项目:国家重点研发计划项目“桉树高效可持续经营技术”（2016YFD0600505） |
|
| Study on the Multiplication and Culture Technology of Eucalyptus urophylla × E. grandis M8 Clones |
| linhua,denghaiyan,zengqi,moxiaoyong |
| (Zhonglin Group Leizhou Forestry Bureau Co. LTD;College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong) |
| Abstract: |
| In order to investigate the effects of minimal medium, 6-BA, IBA, sucrose and pH on the
multiplication of Eucalyptus urophylla × E. grandis M8 clones, an excellent clonal single plant, and its semiligniated shoots were selected as explants. The results showed that :(1) when the medium of MS + 6-BA 0.2 mg/L
+ sucrose 30 g/L + carrageenan 6.8 g/L with pH 5.8 was used to induce the axillary buds of M8 explants, the
germination peak was observed at 10-15 days, with an average pollution rate of 15.3% and an average induction
rate of 77.9%. (2) the optimal medium for M8 multiplication was the improved MS medium, with a concentration
of 0.2-0.5 mg /L for 6-BA, 0.05-0.2 mg /L for IBA, 30 g/L for sucrose, a pH range of 5.4-6.0, a subculture period
of 20 days, which could get a multiplication coefficient of 4.49, and an effective number of 30 shoots per bottle.
Therefore, MS + 6-BA 0.2 mg /L + sucrose 30 g /L + carrageenan 6.8 g /L is the appropriate induction medium
for M8, which can realize rapid axillary bud germination, low pollution rate and high induction rate. The optimal
multiplication medium is improved MS + 6-BA 0.2 mg /L + IBA 0.1 mg /L + sucrose 30 g/L + carrageenan6.8 g/L with a pH of 5.4-6.0, which could realize the extension of seedling leaves and robust growth, improve
tissue culture efficiency and give full play to the advantages of tissue culture and rapid propagation. |
| Key words: Eucalyptus, tissue culture, proliferation, culture medium, 6-BA, IBA |
