| 摘要: |
| 竹材木质素的降解一直是制约竹材资源利用的重要影响因素。文章通过对49 份腐烂的竹材样品进行平板划线分离纯化得到74 株真菌，利用PDA- 愈创木酚变色及PDA- 苯胺蓝退色反应，初步筛选出具有木质素降解酶能力的4 株木质素降解菌，通过酶活力测定对初筛得到的4 株木质素降解菌进行复筛，发现FG-22 菌株具有较好木质素降解能力，对FG-22 降解菌株进行形态学和分子生物学鉴定，确定FG-22 为杂色云芝（Trametes versicolor）。 |
| 关键词: 木质素降解菌 竹材 筛选 鉴定 |
| DOI： |
| 投稿时间：2019-12-30修订日期：2019-12-30 |
| 基金项目: |
|
| Screening and Identification of Bamboo Lignin Degradation Fungus |
| LIU Jian,LI Nanlin |
| (Zhaoqing Forestry Bureau Forest Pest Control and Quarantine Station,Zhaoqing) |
| Abstract: |
| The degradation of bamboo lignin has always been an important factor influencing the utilization of bamboo resources. Seventy-four fungi were isolated by streaking and purifying 49 samples of rotten bamboo. The lignin-degrading fungi producing lignin-degrading enzyme activity were screened by PDA-guaiacol phenol discoloration and PDA-aniline blue fading reaction. Four strains of lignin-degrading fungi were screened by enzyme activity assay. It was found that FG-22 fungus had better lignin degradation ability, and the FG-22-degrading fungus was identified by morphological identification and molecular biology to determine FG-22 is Trametes versicolor. |
| Key words: lignin-degrading fungus bamboo screen identification |
