| 摘要: |
| 为优化水提醇沉法提取油茶 (Camellia oleifera) 饼粕多糖工艺条件，在单因素试验基础上，选
择料液比、提取温度、提取时间为自变量，油茶多糖提取率为响应值，利用 Box-Behnken 中心组合试
验和响应面分析法，研究各自变量交互作用及其对油茶多糖提取率的影响。结果表明，油茶多糖提取最
佳提取工艺为料液比 1:25（g/mL）、提取时间 78 min、提取温度 60 ℃，在此条件下油茶多糖提取率为
（11.29 ± 0.20）%，与预测值偏差 0.18 个百分点，因此优化后回归方程对预测水提醇沉法制备油茶多糖的
提取率分析具有可靠性，三因素中料液比具有显著性影响。 |
| 关键词: 油茶饼粕多糖 水提醇沉法 提取率 响应面分析 |
| DOI： |
| 投稿时间：2019-07-15修订日期：2019-09-18 |
| 基金项目:广东省林业科技创新项目（2017KJCX003）、广东省级科技计划项目（2016A040401008）、广东省级科技计划项目（2015B020202002） |
|
| Optimization of Water Extraction and Alcohol Precipitation Method for Preparation of Camellia oleifera Cake Polysaccharides by Response Surface Analysis |
| zhangmengyu,wangjing,zhang yingzhong,wangminghuai,xubaohua,tangxiaoxu |
| (Guangdong Academy of Forestry) |
| Abstract: |
| In order to optimize the water extraction and alcohol precipitation method for preparation of
Camellia oleifera cake polysaccharides, on the basis of single factor experiment, the ratio of material to liquid,
extraction temperature and extraction time were chosen as independent variables, while the extraction rate of C.
oleifera polysaccharides as corresponding values. With the Box-Behnken center combined test and response surface
analysis, the interaction of each independent variable and its effect on the extraction rate of C. oleifera polysaccharide
were studied. The results showed that the optimum for C. oleifera polysaccharides preparation by water extraction
and alcohol precipitation were 1:25(g/mL) of the ratio of solid to liquid, 78 min of the extraction time, and 60 °C of
the extraction temperature. Under these conditions, the extraction rate of C. oleifera polysaccharides was (11.29 ±
0.20)%, with a deviation of 0.18 percentage points from the predicted value. Therefore, this optimized regression
equation has the reliability of predicting the extraction rate of C. oleifera polysaccharides by the water extraction and
alcohol precipitation method, and the ratio of solid to liquid has a significant effect in the three factors. |
| Key words: Camellia oleifera cake polysaccharides water extraction and alcohol precipitation extraction rate response surface methology |
