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| 一株淡紫紫孢菌12ID-1的分离及其对埃及吹绵蚧的致病性 |
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邓嘉茹,孙龙华,毕可可,张劲蔼,唐立鸿,霍立志
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广州市林业和园林科学研究院,广州市林业和园林科学研究院,广州市林业和园林科学研究院,广州市林业和园林科学研究院,广州市林业和园林科学研究院,广州市林业和园林科学研究院
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| 摘要: |
| 从埃及吹绵蚧虫（Icerya aegyptiaca）体中分离出一株虫生真菌菌株 12ID-1，对其进行了形态观察和 rDNA ITS 序列分析，同时优化了该菌株的产孢条件和测定了其不同浓度的孢子悬浮液对埃及吹绵蚧 2 龄若虫的致病性。结果表明：根据形态特征和分子鉴定结果，该菌株 12ID-1 为淡紫紫孢菌（Purpureocillium lilacinum）；菌株 12ID-1 在 SMAY 培养基、28 ℃和光暗交替（14 L : 10 D）的条件下产孢能力最强；致病性结果表明，当孢子浓度高于 1×106 孢子 /mL 时，随着 12ID-1 孢子浓度的增加，埃及吹绵蚧的校正死亡率越高，LT50 值递减，在孢子浓度为 1×108 孢子 /mL 时，第 7 天校正死亡率接近 100%， LT50 为 3.99 d；随着接种后时间的延续，LC50 值递减，在接种后 7 d，LC50 值为 1.3×106 孢子 /mL。可见淡紫紫孢菌 12ID-1 对埃及吹绵蚧具有较强的毒力，在埃及吹绵蚧生物防治中具有广阔的应用前景。 |
| 关键词: 埃及吹绵蚧 虫生真菌 淡紫紫孢菌 分离鉴定 毒力 |
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| 基金项目:广东省省级乡村振兴战略专项(森林资源培育及管护)项目(2018KJCX054)*通信作者(Author for correspondence),E-mail53584150@qq.com |
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| solation of Purpureocillium lilacinum12ID-1 and Its Virulence against Icerya aegyptiaca |
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dengjiaru,sunlonghua,bikeke,zhangjingai,Tanglihong and huolizhi
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Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou Institute of Forestry and Landscape Architecture,Guangzhou Institute of Forestry and Landscape Architecture
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| Abstract: |
| A fungal strain named12ID-1, was isolated from Icerya aegyptiaca, which was strongly virulent against I.aegyptiaca. The morphological characters and rDNA ITS sequence were examined and analyzed, in the meanwhile,the condition of sporulation production and test the virulent effect of 12ID-1 against the 2nd instar nymph of I. aegyptiaca were optimized. The results were showed as follows: 12ID-1 was identified as Purpureocillium lilacinum based on its morphological characters and rDNA ITS sequence analysis. The optimum solid medium, temperature and light conditions for the growth and spore production of 12ID-1 were Sabouraud maltose agar supplemented yeast extract (SMAY), 28 ℃ , alternation of light and darkness（14L:10D)respectively. The virulence test indicated that the corrected mortality of I. aegyptiaca between concentrations increased with increment of concentration when the spore concentration was above 1×106 conidia/mL, while the values of LT50 were shortened.The corrected mortality was nearly 100% and the LT50 values was 3.99 d at a concentration of 1×108 conidia/mL. The estimates of the median lethal concentration (LC50) decreased with observation days, the estimates of LC50 on day 7 after inoculation was 1.3×106 conidia/mL. It is thus clear that P. lilacinum 12ID-1 has high virulence to I. aegyptiaca, and 12ID-1 could be suitable for application in biocontrol of I. aegyptiaca. |
| Key words: Icerya aegyptiaca entomopathogenic fungus Purpureocillium lilacinum isolation and identification virulence |
