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| 河北省长针线虫新纪录种松长针线虫Longidorus pinus Xu, Ye, Wang & Zhao的形态与分子鉴定 |
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王宏洪,邵晓勇,李秀芬,廖金铃
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广东生态工程职业学院植物有害生物防控与生物环境健康技术研究中心,河北省大名县林业局,广东生态工程职业学院,广东生态工程职业学院植物有害生物防控与生物环境健康技术研究中心
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| 摘要: |
| 2018 年，在河北省保定市园林植物紫荆（Cercis chinensis）根围分离到一种长针线虫。经形态
学观察和 28S rDNA D2D3 区序列分析，将其鉴定为松长针线虫（Longidorus pinus Xu, Ye, Wang & Zhao）。
其雌虫主要形态特征为：雌虫体长 3 048~3 464 μm，唇区明显缢缩，宽 9.5~10.5 μm，侧器囊袋状，齿尖
针长 66.0~69.5 μm，导环距体前端 27.5~30.0 μm，尾长 31~33 μm，短圆锥形，尾长与肛门处体宽比值＝
1.5~1.6。松长针线虫河北种群 28S rDNA D2D3 区与 GenBank 数据库的山西种群进行序列比对，相似性为
99.2%~99.9%。松长针线虫是河北省长针线虫新纪录种。紫荆是松长针线虫的新寄主。 |
| 关键词: 长针线虫 紫荆 形态学 28S rDNA D2D3 区 河北省 |
| DOI： |
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| 基金项目:广东省林业科技创新专项资金项目S“广东省园林植物线虫检测与防控技术研究”(2015KJCX045)。 |
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| Morphological and Uolecular Identification of A New Record of Longidorus pinus Xu, Ye, Wang & Zhao in Hebei Province |
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WANG Honghong,SHAO Xiaoyong,LI Xiufen and LIAO Jinling
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Research Center of Plant Pest Management and Bioenvironmental Health technology,Eco-Engineering Polytechnic,Forest Bureau of Daming County,Guangdong Eco-Engineering Polytechnic,Research Center of Plant Pest Management and Bioenvironmental Health technology,Eco-Engineering Polytechnic
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| Abstract: |
| A Longidorus species was isolated from Cercis chinensis in Baoding city, Hebei province in
2018. The nematode was identified as Longidorus pinus by using morphological observation and 28S rDNA D2D3
sequence analysis. It was characterized by females 3 048-3 464 μm long, lip region distinctly offset from the body
contour (9.5-10.5 μm wide), amphidial fovea pocket-shaped, odontostyle 66.0-69.5 μm long, guide ring located 27.5-
30.0 μm from anterior end, tail short and conoid (31-33 μm long, and c’ = 1.5-1.6. 28S rDNA D2D3 sequence of
L. pinus in Hebei showed high similarities from 99.2% to 99.9% with those sequences in Shanxi. L. pinus is a new
record species of needle nematode in Hebei province. And C. chinensis is a new record host of L. pinus. |
| Key words: needle nematode Cercis chinensis morphology 28S rDNA D2D3 sequence Hebei province |
