| 摘要: |
| 为了探索植物病害生物防治的新途径，对枯草芽孢杆菌（Bacillus subtilis）STO-12 的脂肽类
物质的抑菌活性和特性进行分析，采用滤纸片法、排油试验、乳化试验和表面张力测试检测脂肽类物质
的抑菌活性和特性。研究结果表明，枣缩果病菌（Alternaria tenuissima）、杨树炭疽病菌（Colletotrichum
gloeosporioides）和杨树腐烂病菌（Cytospora chrysosperma）的生长受到脂肽类物质的强烈抑制，最小抑
菌浓度（MIC）分别为 0.02, 0.04 和 0.02 mg·disc-1；分别在高温处理、强酸强碱处理、蛋白酶处理、紫外
线处理及室温保存条件下， STO-12 的脂肽类物质仍能保持其抑菌活性的稳定；另外，通过 3 种不同的检
测试验，发现 STO-12 的脂肽类物质还具有较好的表面活性剂的特性。综上所述，研究表明枯草芽孢杆菌
STO-12 分泌的脂肽类物质具有高效的抗真菌活性，较好的抑菌活性稳定性和较强的表面活性的特性，作
为生防制剂和生物表面活性剂，在工业和田间应用中具有较大的应用潜力。 |
| 关键词: 枯草芽孢杆菌 抑菌脂肽 抑菌活性 特性 |
| DOI： |
| 分类号: |
| 基金项目:北京市支持中央高校共建项目（2050205）；国家自然科学基金（31470647） |
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| Antifungal Activities and Characterization of Lipopeptides Produced by Bacillus subtilis STO-12 |
|
HUANG Huayi,HUANG Yonghuai,HUANG Huanhua,LIANG Yingmei and TIAN Chengming
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Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization /Guangdong Academy of Forestry,Guangzhou,Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization /Guangdong Academy of Forestry,Guangzhou,Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization /Guangdong Academy of Forestry,Guangzhou,Museum of Beijing Forestry University,Beijing Forestry University,Key Laboratory for Silviculture and Conservation of Ministry of Education College of Forestry,Beijing Forestry University,Beijing ,China
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| Abstract: |
| In order to explore new pathways for biocontrol of plant diseases, the antifungal activity and
characterization of the lipopeptides produced by Bacillus subtilis STO-12 have been investigated. Filtering
paper method, oil displacement test, emulsification activity test, and surface tension measurements were
used to determine the antifungal activity and characterization of the lipopeptides. The results indicated that
Alternaria tenuissima, Colletotrichum gloeosporioides, and Cytospora chrysosperma were strong inhibited by
the lipopeptides, the minimum inhibitory concentrations (MICs) were revealed 0.02, 0.04, and 0.02 mg·disc-1.
Even after extreme temperature, pH, and protease treatments, UV radiation, and prolonged storage at room
temperature, the antifungal activity of the lipopeptides remained stable. Moreover, the powerful biosurfactant
properties of the lipopeptides had been found, as assessed by three different methods. This study demonstrates that antifungal lipopeptides secreted by B. subtilis STO-12 possess strong antifungal activity, good stability, and
powerful biosurfactant properties. They may be useful as biocontrol agents and biosurfactants and have potential
in industrial and field applications |
| Key words: Bacillus subtilis antifungal lipopeptides antifungal activity characterization |
