| 摘要: |
| 以绿僵菌素A（DxtA）和绿僵菌素B（DxtB）的产量为指标，分析了僵菌属5个种15个菌株液体培养的产毒素能力差异，确定了高产毒素优良菌株，并拟合了高产毒菌株在摇瓶条件下的产毒素动态。结果表明，不同种绿僵菌间的产毒素能力差异极显著（P＜0.01），黄绿绿僵菌（Metarhizium flavoviride）、戴氏绿僵菌（M. taii）和蚱蜢绿僵菌（M. acridum）均不产生毒素，鳞腮绿僵菌（M. lepidiotae）可产少量毒素，金龟子绿僵菌（M. anisopliae）产毒素能力最强，其中M19的DxtA 产量最高（144.12 μg·mL-1），M25菌株的DxtB产量最高（65.31 μg·mL-1）；M25菌株的综合表现最佳；M25菌株在液体摇瓶培养条件下DxtA产量经SPSS拟合，符合Logist模型：Y=84.99/(1 11.82×e-0.3X），即前4 d是营养生长，毒素分泌量较少，4 d后生物量维持平衡，第5天起毒素产量进入指数增长期，9 d后毒素产量进入平台期。 |
| 关键词: 绿僵菌 毒素 动态曲线 |
| DOI： |
| 分类号: |
| 基金项目:林业公益性行业科研专项“新型高效绿僵菌生物杀虫剂研究”（201304408）。 |
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| Production and Dynamic of Destruxins from Metarhizium spp. |
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Xu Jinzhu and Qin Changsheng
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Guangdong Academy of Forestry,Guangdong Academy of Forestry
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| Abstract: |
| Taking the yield of DxtA and DxtB as indexes, the difference of the destruxin producing ability of 15 Metarhizium strains (belonged to 5 species) was analyzed. The DxtA dynamic curve of the best strain was fitted under shaking flask culture by sampling every day. The results showed that there was a significant difference in the destruxin producing ability among 5 species. Metarhizium anisopliae strains showed highest production, while M. flavoviride, M. taii and M.acridum did not produce DxtA or DxtB, M. lepidiotae produce a little destruxin. Among them, the M19 strain was the best to produce DxtA (144.12 μg·mL-1), and the M25 strain was the best to produce DxtB (65.31 μg·mL-1). The M25 was selected to be the best in DxtA and DxtB production. Under shaking flask culture, the DxtA production of M25 was fitted by SPSS, and showed to accord with Logist model Y=84.99/ (1 11.82×e-0.3X）: produce a little destruxin in the first 4 days vegetative growth and from the fifth day，it change into exponential growth and reached platform period from the ninth day. |
| Key words: Metarhizium destruxin dynamics curve |
