| 摘要: |
| 文章以尾叶桉(Eucalypt urophylla)优良新无性系的无菌组培苗为材料，MS培养基为基本培养基，研究6-BA、NAA不同质量浓度配比对尾叶桉无菌幼苗丛芽增殖率，以及生根粉种类、质量浓度、不同IBA和NAA配比对不同尾叶桉无性系生根率的影响。试验结果表明：当6-BA质量浓度为0.01 mg/L，丛芽增殖率在NAA质量浓度为0.01、0.1 mg/L内递增；而NAA质量浓度为1、5 mg/L时，则有利于生根；筛选出适宜尾叶桉优良无性系ZQUA10增殖培养基为MS 0.01 mg/L 6-BA 0.01 mg/L NAA 30 g/L蔗糖 6 g/L卡拉胶，增殖系数达到4.7；筛选出适宜尾叶桉优良无性系ZQUA13的生根培养基为1/2MS 0.5 mg/L ABT1 30 g/L蔗糖 6 g/L卡拉胶，生根率达到57.5%；筛选出适宜尾叶桉优良无性系ZQUB58生根培养基为1/2MS 1 mg/L IBA 0.5 mg/L NAA 30 g/L蔗糖 6 g/L卡拉胶，生根率达到100%。 |
| 关键词: 尾叶桉 组织培养 再生体系 |
| DOI： |
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| 基金项目:广东省省级科技计划项目“广东主要商品林种业关键技术研究”（2015B070701009）。 |
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| The Research of Eucalypt urophylla on Tissue Culture and Rapid Propagation |
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Liao Huanqin,Huang Xiaoling,Pan Wen and ZHANG Weihua
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Guangdong Academy of Forestry,Zhongkai University of Agriculture and Engineering,Guangdong Academy of Forestry,Guangdong Academy of Forestry
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| Abstract: |
| The paper studied the effects of different 6-BA and NAA concentrations on aseptic seedling cluster buds proliferation rate as well as the effects of different rooting powder and concentration on rooting rate of Eucalyptus urophylla tissue culture seedling with MS medium as basic medium. The study aimed to provid theoretical guidance for the establishment of efficient tissue culture and rapid propagation system and accelerat the efficient utilization of fine varieties and factory?production of E. urophylla. The results showed: With the treatment 0.01 mg/L 6-BA and 0.01，0.1 mg/L NAA, the cluster buds proliferation rate reached the highest; it was beneficial to the growing of roots when NAA concentration was 1，5 mg/L; the proper medium for clonal?variety of E. urophylla(ZQUA10 ) was MS 0.01 mg/L 6-BA 0.01 mg/L NAA 30 g/L sucrose 6 g/L carrageenan, the proliferation coefficient reached 4.7. The proper medium for clonal?variety?of E. urophylla (ZQUA13) was 1/2MS 0.5 mg/L ABT1 30 g/L sucrose 6 g/L carrageenan, the rooting rate reached 57.5%. The proper medium for clonal?variety?of E. urophylla (ZQUB58) was 1/2MS 1 mg/L IBA 0.5 mg/L NAA 30 g/L sucrose 6 g/L carrageenan and the rooting rate reached 100%. |
| Key words: Eucalyptus urophylla tissue culture regeneration system |
