| 摘要: |
| 以洋紫荆（Bauhinia variegata）成熟种子为试验材料，采用质量浓度为0.5、1.0、1.5、2.0 mg/mL 的秋水仙素溶液分别处理12、24、36和48 h，并以蒸馏水浸泡种子作为对照，观察测定其种子萌发特性及幼苗生长指标。结果表明，随着秋水仙素质量浓度的增加，种子发芽率逐渐降低，2.0 mg/mL溶液使大部分种子致死，0.5、1.0 mg/mL质量浓度秋水仙素处理24 h有利于种子萌发；种子胚根的伸长随着秋水仙素质量浓度的增加也受到不同程度地抑制；秋水仙素对发芽种子胚芽的发育有一定的抑制作用；不同质量浓度秋水仙素处理后第7个月，各处理间苗高与地径差异均极显著（P<0.01），质量浓度为1.0 mg/mL，处理36 h时，苗高和地径都最大；当秋水仙素质量浓度为2.0 mg/mL时，对叶片平均分裂角度有一定促进作用。 |
| 关键词: 洋紫荆 秋水仙素 诱变育种 生长指标 |
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| 基金项目:广东省林业科技创新项目“优良乡土阔叶树种无性快繁技术开发与产业化”（2014KJCX002） |
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| Effects of ColchicineTreatment on the seed germination and seeding growth of Bauhinia variegate |
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xujiaqi and xiruchun
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South China Agriculture University,South China Agriculture University
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| Abstract: |
| The mature seeds of Bauhinia variegate were studied by treating with 0.5, 1.0, 1.5 and 2.0 mg/mL colchicines solution for 12, 24, 36 and 48 h respectively, and the seeds soaked with distilled water were taken as control. The germination rate and the mutant seedling growth were observed after the treatments. The results showed that with the increase of colchicines concentration, seed germination rate decreased. 2.0 mg/mL colchicines solution caused death of the seeds, and 0.5,1.0 mg/mL colchicines solution treatment for 24 h was the most beneficial to the germination of the seeds. With the increase of colchicines solution concentration, the radicle elongation was inhibited in different degrees; colchicine could cause the?inhibition effect on continue development of germinating seed embryo. The change of seedling ground diameter was consistent with that of the seedling height after 7 months later, it showed that after soaking the seeds for 36 h in colchicine solution (1.0 mg/mL), the seedling ground diameter and height were both the highest. High concentration（2.0 mg/mL） colchicine solution on average leaf split angle played an important role in germination promoting. The stimulative effect on leaf angle was found when the concentration of colchicines solution was 2.0 mg/mL. |
| Key words: Bauhinia variegate colchicine mutation breeding growth index |
